The preliminary study of the differential diagnosis of ascites using Golgi glycoprotein 73 and alpha fetal protein / 中华检验医学杂志

Objective To investigate the concentration of Golgi glycoprotein 73(GP73) in ascites, then study the value of GP73 and alpha fetal protein(AFP)in the differential diagnosis of ascites.Methods Totally 96 malignant ascites specimens , 35 benign ascites specimens and their paired serum specimens were collected in the Tumor Hospital Affiliated to Xinjiang Medical University from November 2012 to November 2013.GP73 in serum and ascites were detected by ELISA and AFP in ascites was measured by electrochemical luminescence.The application values of GP 73 and AFP were evaluated through ROC curve.Results In benign ascites group , the difference of GP73 between the ascites ( 16.06 ±9.53 ) ng/ml and the serum (13.69 ±8.87) ng/ml was statistically significant (t=5.026,P<0.001), which showed a good linear correlation (r=0.966,P<0.001).In malignant ascites group, the difference between the ascites (28.37 ±12.02) ng/ml and the serum (16.06 ±9.53) ng/ml was statistically significant (t=10.641,P<0.001), and also showed a good linear correlation (r=0.933,P<0.001).The cutoff values of GP73, AFP and GP73*AFP (GP73 multiplied AFP) for diagnosing the malignant ascites were 17.1 ng/ml, 13.1 ng/ml and 321.The area under ROC curve ( AUC) were 0.795, 0.753 and 0.902 respectively.The AUC of GP73*AFP is the largest.The sensitivity of three index diagnosing the ascites , were 89.6%( 86/96 ) , 88.5%(85/96) and 86.4%(83/96); and the specificity were 60.0%(21/35), 54.2%(19/35) and 85.7%( 30/35 ) respectively.Conclusions Detection of GP73 in ascites specimens has clinical value , GP73*AFP was the best indicators for differential diagnosis of benign and malignant ascites compared to GP73 and AFP.

Evaluate the effect of CO2 laser-assisted uvulopalatopharyngoplasty for obstructive sleep apnea-hypopnea syndrome / 临床耳鼻咽喉头颈外科杂志

OBJECTIVE@#To seek the effect of CO2 laser-assisted uvulopalatopharyngoplasty (LAUP) of obstructive sleep apnea hypopnea syndrome (OSAHS).@*METHOD@#Patients with OSAHS confirmed by polysomnography (PSG) were chosen. Ninety-six patients were completed PSG studies of more than 6 months after CO2 LAUP.@*RESULT@#The 96 patients' follow-up period was 6 months, 29 cases were cured; 34 cases had notable effects; 13 cases had effects; 20 cases had no effects. The effective rate was 79.17% (76/96).@*CONCLUSION@#Application CO2 LAUP is a safe and effective treatment method for OSAHS.

Gene expression profiles in human nasal polyps studied by DNA microarray / 临床耳鼻咽喉头颈外科杂志

OBJECTIVE@#To explore the changes in gene expressions in the human nasal polyp.@*METHOD@#A 14500 gerie DNA microarray (Affymetrix) was used to examine gene expressions in 6 NP samples, 6 normal mucosal samples. The differentially expressed genes were identified and subjected to realtime PCR analysis.@*RESULTS@#The differentially expressed genes mostly involved in cytokines, adhesion genes about complements and their receptors, immune transcription regulatory molecules, signal transduction, differentially expressed genes including IL-8, RGS1, GRK4, CCL20, uteroglobin. The results of IL-8, RGS1 Real-time PCR of were consistent with that of gene chip analysis.@*CONCLUSION@#Microarray expression profile of NP samples is differential. RGS1 may play an important role in cell signal transduction, IL-8 may involve in occurrence of nasal polyps by inducing cells releasing inflammatory factors.