Identification of main chemical constituents in extracts of Akebiae Fructus by HPLC-ESI-MS / 中草药

Objective: To establish an HPLC-ESI-MS method for quickly identifying the chemical constituents in the extracts of Akebiae Fructus. Methods: The main saponin components in the extracts of Akebiae Fructus were detected with the HPLC-ESI-MS in negative ion mode. These components were further analyzed by MS2 and MS3 spectra, and by comparing with the corresponding reference substances and literature data. Results: Seventeen saponins in the extracts of Akebiae Fructus were well separated in one run. Conclusion: The new method is accurate and rapid. It could be used to identify the main chemical constituents in the extracts of Akebiae Fructus and be suitable for the quality control of Akebiae Fructus.