Comparative Study of the Two High-Efficient Strategies for in vitro Generation of Human Umbilical Cord Blood-derived Natural Killer Cells / 中国实验血液学杂志

OBJECTIVE@#To explore the similarities and variations of biological phenotype and cytotoxicity of human umbilical cord blood natural killer cells (hUC- NK) after human umbilical cord blood-derived mononuclear cells (hUC-MNC) activated and expanded by two in vitro high-efficient strategies.@*METHODS@#Umbilical cord blood mononuclear cells (MNC) from healthy donor were enriched by Ficoll-based density gradient centrifugation. Then, the phenotype, subpopulations, cell viability and cytotoxicity of NK cells derived from Miltenyi medium (denoted as M-NK) and X-VIVO 15 (denoted as X-NK) were compared using a "3IL" strategy.@*RESULTS@#After a 14-day's culture, the contents of CD3-CD56+ NK cells were elevated from 4.25%±0.04% (d 0) to 71%±0.18% (M-NK) and 75.2%±1.1% (X-NK) respectively. Compared with X-NK group, the proportion of CD3+CD4+ T cells and CD3+CD56+ NKT cells in M-NK group decreased significantly. The percentages of CD16+, NKG2D+, NKp44+, CD25+ NK cells in X-NK group was higher than those in the M-NK group, while the total number of expanded NK cells in X-NK group was half of that in M-NK group. There were no significant differences between X-NK and M-NK groups in cell proliferation and cell cycle, except for the lower percentage of Annexin V+ apoptotic cells in M-NK group. Compared with X-NK group, the proportion of CD107a+ NK cells in M-NK group were higher under the same effector-target ratio (E∶T) (P<0.05).@*CONCLUSION@#The two strategies were adequate for high-efficient generation of NK cells with high level of activation in vitro, however, there are differences in biological phenotypes and tumor cytotoxicity.

Research progress in cytoplasmic PML gene functions / 中国实验血液学杂志

The promyelocytic leukemia (PML) was originally identified and named as acute promyelocytic leukaemia (APL) . The PML, encoded by PML gene, locates in the nuclear body (NB) and shuttles in the cell nucleus-cytoplasm, so that PML completes many regulation functions. There are many research on the function of nuclear PML, but in recent years the foreign data indicate that cytoplasmic PML gene plays an important role in hematologic malignancies and solid tumors. In this article, the biological functions of PML gene in cytoplasm are reviewed.

The effect of DLX4 on the apoptosis of choriocarcinoma cells and its related mechanism / 肿瘤

Objective: To explore the effect of homeodomain protein DLX4 on apoptosis of choriocarcinoma cells and its related mechanism. Methods: RNA interference (RNAi) was used to knockdown DLX4 expression in JEG-3 cells. Then the inhibitory effect of DLX4 on apoptosis was determined by flow cytometry and the expression of apoptosis-related protein cleaved caspase-3 and caspase-8 and bax was detected by western blotting. Results: RNA interference targeted for DLX4 effectively and specifically inhibited DLX4 mRNA expression and decreased DLX4 protein level in JEG-3 cells. The apoptotic rate of JEG-3 cells was increased, the expression levels of cleaved both caspase-3 and caspase-8 were elevated but the bax protein expression did not change after knocking down DLX4. Conclusion: RNA interference silences DLX4 expression and induces apoptosis of JEG-3 cells. The mechanism for apoptosis was related with transcriptional regulation on caspase-3 and caspase-8.