Résumé
By taking doctors looking for a job in a certain city of Shandong Province as the research object, this paper to explore the career cognition influence path and degree of career intention among general practitioners.After building a general practitioners' career cognition and intention analysis framework, and conducting random sampling among doctors, this paper uses factor analysis to obtain factor scores and adopts structural equation model to conduct analysis and verification, by taking career cognition as independent variables, career identity and income expectation as intermediary variables, career intention as dependant variables.The empirical results show that the career cognition affects income expectation, which in turn affects career recognition, and the indirect effect on career intention is ultimately also significant.In practice, as these results help to understand the relationship between these variables, the government should consider the impacts of policy on career cognition among general practitioners when formulating policies.
Résumé
Health policy research could provide prospecting and effective references and suggestions for top-level design of the new medical reform.Advocacy Coalition Framework was a very important model for the research policy change that synthesizes institutional economics,political science and other theories,which had strong explanatory power in many policy areas of high-income countries.However,China was still in its infancy.It introduced the main ideas of the framework,development,application status of health policy field at home and abroad,and put forward the future research direction in the field of health policy that in order to promote the application of the framework in health policy research in China.improve the theoretical and scientific levels of the policy research.
Résumé
Objective Increasing evidence suggest that aberrant activation of PI3K/Akt is involved in many human cancers, and that inhibition of the PI3K/Akt pathway might be a promising strategy for cancer therapy. The study is to evaluate the effects of combined therapy of PI3K inhibitor (LY294002) and Ad-PTEN in athymic mice xenogeneic transplant model of human glioma and to reveal the possible mechanisms involved.Methods Twenty-four athymic mice were randomly divided into 4 groups (DMSO、Ad-vector plus DMSO、LY294002 alone and Ad-PTEN plus LY294002), and were treated, respectively. Athymic mice xenogeneic transplant model was established by inoculation (sc) with LN229 glioma cells. Body mass (BM) and diameter of tumor mass were measured. Furthermore, The protein expressions of PTEN、p-Akt、CyclinD1、Caspase-3、MMP-2、p-FAK in tumor tissues were analyzed with immunohistochemistry.Results The tumor-inhibiting rate of was significantly higher in Ad-PTEN plus LY294002 than in the LY294002 alone (92.46 vs 65.59%)( P <0.05).The protein expressions of PTEN and Caspase-3 were significantly higher, while PCNA、CyclinD1、bcl-2 and MMP-2、p-FAK was significantly lower in Ad-PTEN plus LY294002 group than in the other three groups ( P <0.05).Conclusions LY294002 plus Ad-PTEN achieve better outcome than either alone in treating glioma possibly through enhancement of the inhibitory action of PI3K/Akt pathway and Ad-PTEN pathway.