Effects of growth stages and pH value on the expression of autolytic enzyme atIS gene of Streptococcus gordonii / 华西口腔医学杂志

<p><b>OBJECTIVE</b>This study aimed to detect the difference in the expression levels of autolysin atIS gene of Streptococcus gordonii (S. gordonii) at different growth stages and pH values, as well as to analyze the factors regulating atlS gene expression in S. gordonii.</p><p><b>METHODS</b>S. gordonii wild strains (ATCC 35105) were collected at different growth stages (early exponential phase, mid-exponential phase, late exponential stage, and platform stage) and pH values (pH 7 and pH 5.5), and total RNA was extracted by using a conventional method. Fluorescence quantitative polymerase chain reaction (FQ-PCR) was used to measure the relative mRNA expression of atlS gene, with bacterial 16S rRNA as internal reference, for a comparison of the mRNA levels of atlS gene expression in S.gordonii at different growth stages and pH values.</p><p><b>RESULTS</b>FQ-PCR results showed that atlS gene expression increased with gradually increasing growth stage under neutral conditions and was higher than that under acidic conditions (P < 0.05).</p><p><b>CONCLUSION</b>The atlS gene expression in S. gordonii is influenced by growth stage and pH value factors.</p>

Effect to demineralization and remineralization of enamel surface by fluorine / 华西口腔医学杂志

<p><b>OBJECTIVE</b>To analyze the mechanism of fluorine by systemic analysis of fluorination-demineralization-remineralization experiments.</p><p><b>METHODS</b>The enamel specimens were randomly assigned to untreated group (group A), non-fluoride group (group B), low-fluoride group (group C) and high-fluoride group (group D). The in vitro model of fluoride enamel was established in group C and D. Based on that, the establishment of demineralization model and remineralization experiment by pH-cycling in group B, C and D were followed. All enamel specimens were observed by stereomicroscope and scanning electron microscope and compared in surface microhardness value.</p><p><b>RESULTS</b>There was distinct difference in micro-morphologic appearance on fluoride enamel surface. Artificial caries of fluoride enamel showed a relatively complete surface, the surface microhardness after demineralization and remineralization in fluoride group was higher than non-fluoride group (P < 0.05).</p><p><b>CONCLUSION</b>The fluorinated enamel can enhance cariostatic potential and remineralization capacity of dental enamel.</p>

Practice and Consideration on the construction of digital hospital / 中华医院管理杂志

Digital hospital construction is foundational project for the modern management,which challenges the management mode and idea in hospital.With regard to the status on the construction of digital hospital of infectious disease in 302 hospital of the People's Liberation Army,The article reviews the experience and prospect of digital hospital of infectious disease.