Role of tumor necrosis factor-like ligand 1 aberrance in the immune regulation of inflammatory bowel disease / 中国综合临床

Inflammatory bowel disease includesulcerative colitis and Crohn′s disease.At present, it is generally believed that the pathogenesis of inflammatory bowel disease is that environmental factors act on the genetic susceptible.With the participation of intestinal flora, it starts the intestinal immune response which is difficult to stop and alternates between attack and remission.However, the pathogenesis of IBD is not very clear, so it is important to further explore and clarify the pathogenesis of IBD for the search of new treatment.Tumor necrosis factor-like ligand 1 aberrance(TL1A) is a newly discovered new member of tumor necrosis factor family and a susceptible gene of inflammatory bowel disease.TL1A plays a key role in the regulation and connection between innate immunity and adaptive immunity of mucosal inflammation, and thus plays an important role in inflammatory bowel disease.This article reviews the role of TL1A in the immune regulatory mechanism of inflammatory bowel disease.

Research advances in the role of macrophages in nonalcoholic fatty liver disease / 临床肝胆病杂志

With the improvement of living standard, the incidence rate of nonalcoholic fatty liver disease (NAFLD) is gradually increasing year by year and its age of onset tends to become younger, but its pathogenesis remains unclear. Macrophages are important cells involved in the pathogenesis of NAFLD and have attracted great attention. This article elaborates on the origin and classification of liver macrophages, the role of macrophages in liver inflammation and related activation mechanism, and the drugs targeting macrophages, in order to provide a reference for the clinical treatment of NAFLD.

The effects of macrophages with high expression of TL1A on activation and proliferation of hepatic stellate cells in vitro / 中华肝脏病杂志

Objective@#To explore the effects of macrophages with high expression of TL1A on the activation and proliferation of HSCs in vitro. @*Methods@#The Bone marrow-derived macrophages (BMMs) and peritoneal macrophages (PMs) from wild type (WT) and myeloid-overexpressed TL1A transgenic mice were isolated, differentiated and activated. HSCs were harvested from activated macrophages culture supernatant (CM). HSCs were detected by immunofluorescence and real-time Q-PCR. And the proliferation was detected by CCK-8 and BrdU assay kit. The levels of IL-1β and PDGF-BB in macrophage culture supernatants were determined by enzyme-linked immunosorbent assay (ELISA). @*Results@#BMMs-derived CM-intervention HSCs were used to detect the expression of α-smooth muscle actin (α-SMA) on the 2nd, 4th and 6th day respectively by immunofluorescence method. There was no significant difference between the two groups on the 2 nd and the 6th day, P > 0.05; On day 4, the CM/Tg group was significantly higher than that of CM/WT group, P < 0.01; the results of CMs derived from PMs were consistent with the above trend. The expression of α-SMA mRNA on the 2nd, 4th and 6th day was detected by real-time Q-PCR method using BM-derived CMs. No significant difference was found between the groups on the 2nd day (P > 0.05).α-SMA mRNA increased further on the 4th and 6th day, and the level of CM/Tg in CM/Tg group was significantly higher than that in CM/WT group (P < 0.05). The detection results of CMs derived from PMs were consistent with the above trend. The results of CCK-8 assay and BrdU assay showed that the proliferation rate of HSCs in CM Tg group was significantly higher than that in CM/WT group (P < 0.01). The CMs derived from PMs were used to interfere with HSCs. And the results were consistent with the above trend. For BMMs, the levels of IL-1β and PDGF-BB in the lipopolysaccharide (LPS) + IFNγ/Tg culture supernatant were significantly higher than those in the LPS+IFNγ/WT group (P < 0.01). For the culture supernatants of PMs Liquid test results consistent with the above trend. @*Conclusion@#Macrophages with high expression of TL1A could enhance the activation and proliferation of HSCs by increasing the secretion of IL-1β and PDGF-BB.

Research advances in the role of macrophages in nonalcoholic fatty liver disease / 临床肝胆病杂志

With the improvement of living standard, the incidence rate of nonalcoholic fatty liver disease (NAFLD) is gradually increasing year by year and its age of onset tends to become younger, but its pathogenesis remains unclear. Macrophages are important cells involved in the pathogenesis of NAFLD and have attracted great attention. This article elaborates on the origin and classification of liver macrophages, the role of macrophages in liver inflammation and related activation mechanism, and the drugs targeting macrophages, in order to provide a reference for the clinical treatment of NAFLD.