Validation and invalidation of SARS-CoV-2 main protease inhibitors using the Flip-GFP and Protease-Glo luciferase assays

SARS-CoV-2 main protease (Mpro) is one of the most extensively exploited drug targets for COVID-19. Structurally disparate compounds have been reported as Mpro inhibitors, raising the question of their target specificity. To elucidate the target specificity and the cellular target engagement of the claimed Mpro inhibitors, we systematically characterize their mechanism of action using the cell-free FRET assay, the thermal shift-binding assay, the cell lysate Protease-Glo luciferase assay, and the cell-based FlipGFP assay. Collectively, our results have shown that majority of the Mpro inhibitors identified from drug repurposing including ebselen, carmofur, disulfiram, and shikonin are promiscuous cysteine inhibitors that are not specific to Mpro, while chloroquine, oxytetracycline, montelukast, candesartan, and dipyridamole do not inhibit Mpro in any of the assays tested. Overall, our study highlights the need of stringent hit validation at the early stage of drug discovery.

The Adaptation of McLean Screening Instrument for Borderline Personality Disorder Among Chinese College Students / 中国临床心理学杂志

Objective:To revise the McLean Screening Instrument for Borderline Personality Disorder(MSI-BPD)in China and explore the four-factor solution in Chinese non-clinical sample.Methods:A total of 1206 college students(749 males and 457 females) from Peking university at the mean age of 20.02?1.77 completed the measures successfully.Results:The MSI-BPD showed good internal consistency and concurrent validity.Confirmatory factor analysis confirmed the four-factor solution(emotional disregulation,impulsivity,cognitive disturbance,unstable interpersonal relationship) in our sample of Chinese college students.Conclusion:MSI-BPD is a valid measure in Chinese college students.Future research should be done for its use in Chinese clinical samples.

Featwes of educating Medical Students for the Effective Medical Ethics / 中国医学伦理学

In order to fit in will the needs of socialist cultural and ideological progress, medical moral education should be strengtland and ist methods should be improwed. The only way to improve the methods is to liberate the thoughts, reney ideas, from new style ideas and educate students comprehensively for medical morals.