Long-term Helicobacter pylori Infection Does Not Induce Tauopathy and Memory Impairment in SD Rats / 华中科技大学学报（医学）（英德文版）

Helicobacter pylori (H.pylori) infection is a recognized risk factor of dementia,while its role and mechanism in Alzheimer disease (AD) remained unclarified.Our previous study has identified that injection of soluble H.pylori filtrate could induce AD-like pathologic changes and cognitive impairment in SD rats.In the present study,we further explored the effect of long-term stomach colonization of H.pylori bacteria on the brains of SD rats.The results showed that H.pylori bacteria gavage induced an efficient colonization of H.pylori in the stomach after four weeks.However,there was no significant change of tau phosphorylation at Thr205 (pT205),Thr231 (pT231),Ser396 (pS396) and Ser404 (pS404) sites in the hippocampus and cerebral cortex.The H.pylori-infected rats also showed no cognitive impairment.These observations may result from inefficient release of bacterial pathogenic factors or the overall lack of host inflammatory responses.We conclude that SD rat with long-term H.pylori colonization in the stomach is not a suitable animal model for exploring the effects of H.pylori infection on brain function in human beings;administration of bacterial filtrates may better reveal the systemic pathologic changes induced by bacterial infection in animals which show a negative host response to bacterial colonization.

Comparative Study of the Primary Healthcare Systems in China and Mali

"Background: Community health centres (CHCs) are an important component of the health system in Mali and China. Despite patient support and commitment from the authorities; the management and the quality of care of these structures need to be improved. This research aimed to compare the management style of the relevant boards with users' satisfaction pertaining to CHC services in Mali and China. Method: Between September 2009-January 2010; a study was conducted in eight CHCs in Mali and in 16 CHCs in China. A total of 480 householders; [60 (Mali) and 320 (China)]; and 24 management committee members (Mali) and 48 management committee members (China) were interviewed. An in-depth interview technique was used on members of the management committee; while a structured interview was carried out to collect data during face-to-face contact with householders in their residences. In residences in which there were two or more households; the first willing householder was interviewed. Questions about the level of satisfaction were coded from 1-5 and ranged from ""excellent"" to ""very bad"" respectively. Results: The CHCs in China were managed by the government; whereas in Mali; they were overseen by the local population. The most satisfied people in both countries were those living in the poorest socioeconomic conditions; the elderly; and those who attended the healthcare meetings. Chinese households were more satisfied with the quality of the CHC services than those in Mali. The Chinese management boards proposed standardisation of rules and more funding; whereas those in Mali advocated that government provide more funding and human resources allocation. Conclusion: A high level of satisfaction regarding the CHC services was observed. However; users reported on deficits in the quality of care; whereas management suggested a need for greater resource allocation"

Comparative Study of the Primary Healthcare Systems in China and Mali

"Background: Community health centres (CHCs) are an important component of the health system in Mali and China. Despite patient support and commitment from the authorities; the management and the quality of care of these structures need to be improved. This research aimed to compare the management style of the relevant boards with users' satisfaction pertaining to CHC services in Mali and China. Method: Between September 2009-January 2010; a study was conducted in eight CHCs in Mali and in 16 CHCs in China. A total of 480 householders; [60 (Mali) and 320 (China)]; and 24 management committee members (Mali) and 48 management committee members (China) were interviewed. An in-depth interview technique was used on members of the management committee; while a structured interview was carried out to ollect data during face-to-face contact with householders in their residences. In residences in which there were two or more households; the first willing householder was interviewed. Questions about the level of satisfaction were coded from 1-5 and ranged from ""excellent"" to ""very bad"" respectively. Results: The CHCs in China were managed by the government; whereas in Mali; they were overseen by the local population. The most satisfied people in both countries were those living in the poorest socioeconomic conditions; the elderly; and those who attended the healthcare meetings. Chinese households were more satisfied with the quality of the CHC services than those in Mali. The Chinese management boards proposed standardisation of rules and more funding; whereas those in Mali advocated that government provide more funding and human resources allocation. Conclusion: A high level of satisfaction regarding the CHC services was observed. However; users reported on deficits in the quality of care; whereas management suggested a need for greater resource allocation."

Ultrasound-triggered Microbubble Destruction in Combination with Cationic Lipid Microbubbles Enhances Gene Delivery / 华中科技大学学报（医学）（英德文版）

This study aimed to examine the preparation of cationic lipid microbubble (CLM),and evaluate its physical and chemical properties and toxicity,measure the gene transfection efficiency by ultrasound triggered microbobble destruction (UTMD) in combination with CLM.The CLM was prepared by the method of the thin film hydration,and its morphology was observed under the electron microscopy at 1st,3rd,7th,10th,and 14th day after preparation,respectively.The size,Zeta potential and stability of CLM were tested.The acute toxicity of CLM was assessed.The green fluorescent protein gene (EGFP) transfection efficiency was evaluated.The experiment grouping was as follows:naked plasmid group (P group),ultrasonic irradiation plus naked plasmid group (P-US group),naked plasmid plus CLM group (P-CLM group),naked plasmid plus ultrasound and CLM group (UTMD group).The expression of EGFP was detected by fluorescent microscopy and flow cytometry.The results showed that CLMs were spherical in shape,with the similar size and good distribution degree under the light and electron microscopies.The size of CLMs was varied from 250.4±88.3 to 399.0±99.8 nm and the Zeta potential of CLMs from 18.80±4.97 to 20.1 ±3.1 mV.The EGFP expression was the strongest in the UTMD group,followed by the P-CLM group,P-US group and P group.Flow eytometry results were consistent with those of fluorescent microscopy.The transfection efficiency was substantially increased in the P-US group,P-CLM group and UTMD group as compared with that in the P group,almost 7 times,10 times and 30 times higher than that in the P group respectively.It is suggested that CLMs prepared by the method of thin film hydration are uniform in diameter,and proved non-toxic.UTMD combined with CLM can significantly increase the transfection efficiency of EGFP to targeted cells.

Role of Protein Kinase Cin Advanced Glycation End Products-induced Epithelial-Mesenchymal Transition in Renal Proximal Tubular Epithelial Cells / 华中科技大学学报（医学）（英德文版）

d by AGE-BSA may be mediated via the activation of PKC signal transduction pathway.

Redox modulation of large conductance calcium-activated potassium channels in rat cultured trigeminal ganglion neurons / 中国应用生理学杂志

<p><b>AIM</b>To observe redox modulation of ion channel in trigeminal ganglion neurons by oxidants and reducing agents.</p><p><b>METHODS</b>The effects of oxidants and reducing agents on maxi-conductance calcium-activated potassium channel in cultured rat trigeminal ganglion neurons by using whole-cell patch-clamp technique.</p><p><b>RESULTS</b>Methionine-specific oxidant chloramine-T (Ch-T) 1 mmol/L slightly increased the current amplitude and this enhancement did not antagonized by DTT. In contrast, cysteine-specific reagent 5, 5'-dithio-bis(2-nitrobenzoic acid) (DTNB) 500 micromol/L significantly decreased current amplitude of BK(Ca) channels. The effect was reversed by the reducing agent 2 mmol/L 1, 4-dithio-DL-threitol (DTT).</p><p><b>CONCLUSION</b>Reactive oxygen species were definitely involved in regulation of native neuronal function via redox modulation of BK(Ca) channels, which are suggested to play compensatory roles under oxidative stress-related conditions.</p>