Cladosporium cladosporioides isolado de gato com carcinoma de células escamosas / Cladosporium cladosporioides isolated from a cat with squamous cell carcinoma

Este relato descreve o caso do Cladosporium cladosporioides isolado de uma lesão periocular de um felino atendido no Hospital Veterinário da Universidade Federal de Mato Grosso, em Cuiabá. A principal queixa do proprietário era uma lesão periocular, com piora no decorrer do tempo, havia aproximadamente quatro meses. Foi descrita a tentativa de tratamento da lesão com anti-inflamatórios e antibióticos, sem sucesso. Na anamnese foi relatado que o animal tinha acesso à rua e a hábitos de caça e que não havia outros animais da casa com lesão semelhante. O animal foi submetido à biópsia e citologia para um diagnóstico mais preciso do caso. Um fragmento foi encaminhado para o Laboratório de Patologia Veterinária e outro para o de Microbiologia Veterinária. Nas análises histopatológicas, houve compatibilidade com carcinoma de células escamosas e, nas lâminas de citologia, foi evidenciado um processo inflamatório agudo. Nas características macroscópicas e microscópicas da colônia, houve compatibilidade com Cladosporium sp. Posteriormente, o DNA foi extraído e sequenciado, confirmando a espécie Cladosporium cladosporioides. O objetivo deste relato foi descrever o isolamento dessa espécie em um felino com carcinoma de células escamosas.(AU)

This report describes a case of Cladosporium cladosporioides isolated from a cat with a periocular lesion at the Veterinary Hospital, Cuiaba- Brazil. Owner described his animal as having a periocular lesion treated unsuccessfully with anti-inflamatories and antibiotics. During anamnesis, it was reported that the animal has access to the street, hunting habits and none of the other animals of the house had a similar injury. The animal underwent biopsy and cytology for more accurate diagnosis of the case. A fragment was referred to the Veterinary Pathology Laboratory and another for Veterinary Microbiology. In the histopathological analysis of biopsy, it was compatible with squamous cell carcinoma and the cytology slides showed an acute inflammatory process. Microbiogical analysis isolated fungus with Cladosporium sp. Subsequently, DNA was extracted and sequenced confirming Cladosporium cladosporioides species. This paper reports the isolation of this species in a feline with squamous cell carcinoma.(AU)

Characterization of bacteriophages infecting clinical isolates of Pseudomonas aeruginosa stored in a culture collection

Some clinical isolates of Pseudomonas aeruginosa stored in our culture collection did not grow or grew poorly and showed lysis on the culture plates when removed from the collection and inoculated on MacConkey agar. One hypothesis was that bacteriophages had infected and killed those clinical isolates. To check the best storage conditions to maintain viable P. aeruginosa for a longer time, clinical isolates were stored at various temperatures and were grown monthly. We investigated the presence of phage in 10 clinical isolates of P. aeruginosa stored in our culture collection. Four strains of P. aeruginosa were infected by phages that were characterized by electron microscopy and isolated to assess their ability to infect. The best condition to maintain the viability of the strains during storage was in water at room temperature. Three Siphoviridae and two Myoviridae phages were visualized and characterized by morphology. We confirmed the presence of bacteriophages infecting clinical isolates, and their ability to infect and lyse alternative hosts. Strain PAO1, however, did not show lysis to any phage. Mucoid and multidrug resistant strains of P. aeruginosa showed lysis to 50% of the phages tested.