Marrow mesenchymal stem cell transplantation with sodium alginate gel for repair of spinal cord injury in mice / 浙江大学学报·医学版

<p><b>OBJECTIVE</b>To investigate the effects of sodium alginate gels on marrow mesenchymal stem cell transplantation for repair of spinal cord injury (SCI) in mice.</p><p><b>METHODS</b>In the present study, effects of different sterilization methods and concentrations of sodium alginate gels were examined. Marrow mesenchymal stem cells (mMSCs) were isolated from mice and cultured. Cells were cultured with sodium alginate gels and MTT assay was applied to determine the cell viability. Mice spinal cord injury was induced by spinal cord transection. mMSCs were transplanted into the cavity of injured spinal cord with sodium alginate gels. The effects of sodium alginate gel were assessed by BMS scales and immunofluorescence staining for NF-200.</p><p><b>RESULTS</b>Compared with liquid form, solid form sodium alginate gel prepared with high pressure vapor sterilization had a better effect on cell viability. SCI mice treated with 10 % sodium alginate gel and mMSCs achieved higher score in BMS scale as well as higher expression of NF-200 compared with the untreated SCI group.</p><p><b>CONCLUSION</b>Sodium alginate gel prepared with solid form sterilization induces mMSCs proliferation and thus can be used as the carrier of stem cell in treatment of SCI.</p>

Somatostatin mRNA expression in hippocampus of diabetic rats model / 中国医学科学院学报

<p><b>OBJECTIVE</b>To investigate the relationship between diabetes and somatostatin (SOM).</p><p><b>METHODS</b>We established a streptozocin-induced diabetic rats model and studied the changes of SOM mRNA in hippocampi of diabetic and normal rats by using in situ hybridication and computer image analysis. We studied the mechanism of chronic diabetes encephalopathy by observing the changes of somatostatin mRNA in the hippocampus of diabetic rats was studied.</p><p><b>RESULT</b>The number, light density and average area of positive cells in dentate gyrus area of hippocampus in diabetes model were reduced significantly as compared with normal rats (P < 0.01).</p><p><b>CONCLUSION</b>A decline in SOM mRNA expression may play a role in chronic diabetes encephalopathy because of SOM effect in brain.</p>