No association of K469E polymorphism of intercellular adhesion molecule-1 with rheumatoid arthritis / 中华医学遗传学杂志

<p><b>OBJECTIVE</b>To investigate the association of the intercellular adhesion molecule-1 gene (ICAM-1) K469E polymorphism and rheumatoid arthritis (RA).</p><p><b>METHODS</b>Two hundred and seventy-five patients with RA and 254 healthy individuals were collected and enrolled in the study. The K469E polymorphism of ICAM-1 gene was analyzed by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP).</p><p><b>RESULTS</b>The genotype frequencies of KK, KE and EE of K469E polymorphism were 0.535,0.411 and 0.054 respectively in the RA patients, and 0.512,0.437 and 0.051 respectively in the healthy individuals, and there was no significant difference between the two groups (chi² = 0.371, P = 0.831). The frequencies of the K469 allele were 0.74 and 0.73 in the RA patients and the controls respectively (chi² = 0.127, P = 0.721, OR = 1.051, 95%CI:0.800-1.381). No significant difference was observed in KK + KE genotype frequencies between the two groups (P = 0.863), with an odds ratio of 0.935 (95%CI:0.436-2.005).</p><p><b>CONCLUSION</b>The K469E polymorphism of the ICAM-1 gene was not associated with the susceptibility of rheumatoid arthritis.</p>

Clinical significances of combined detections of autoantibodies in patients with systemic lupus erythematosus / 浙江大学学报·医学版

<p><b>OBJECTIVE</b>To evaluate the clinical significances of combined detections of the autoantibodies ANA, ANAs and anti-dsDNA in patients with systemic lupus erythematosus (SLE).</p><p><b>METHODS</b>ANA was tested by indirect immunofluorescent method (IIF), ENA was tested by Western blot and anti-dsDNA was measured with radioimmunoassay in the serum samples from patients with SLE (n=90), and from the comparison group (n=74) and healthy control group (n=53).</p><p><b>RESULTS</b>The sensitivity of anti-dsDNA, AnuA,anti-Sm in patients with SLE was 41.1%, 32.2% and 22.2%. The specificity of anti-dsDNA, AnuA, anti-Sm in SLE patients was all 97.3%. The positive likelihood ratios were 15.22, 11.93 and 8.22, respectively. The sensitivity and specificity reached 62.2% and 90.5%, respectively by combined determinations of anti-dsDNA, AnuA and anti-Sm.</p><p><b>CONCLUSION</b>The combined examinations of anti-dsDNA, AnuA, anti-Sms can improve the sensitivity and efficiency of laboratory diagnosis of SLE.</p>