Further promotion effect of variable temperature drying on effective components in roots of Salvia miltiorrhiza f. alba / 中国中药杂志

To study the effects of different variable temperature drying modes on active components of roots of Salvia miltiorrhiza f. alba, and provide basis for its industrialized drying process. In order to ensure the content of active components, variable temperature drying modes were designed: low temperature at 30 ℃ and high temperature at 60 ℃, low temperature at 30 ℃ and high temperature at 70 ℃, low temperature at 30 ℃ and high temperature at 80 ℃, low temperature at 40 ℃ and high temperature at 60 ℃, low temperature at 40 ℃ and high temperature at 70 ℃, low temperature at 40 ℃ and high temperature at 80 ℃ and air dry oven was used for variable temperature drying process. Then HPLC method was used to determine the changes of active components in roots of S. miltiorrhiza f. alba under different temperature modes; and SPSS 17.0 was used to analyze the data. The results showed that the samples, which were first dried at 40 ℃ for six hours and then dried at 80 ℃ for three hours, had the highest contents in dihydrotanshinone, cryptotanshinone, tanshinone Ⅰ and tanshinone ⅡA as compared with other kinds of drying methods, and the contents were 0.35, 2.76, 0.78, 4.47 mg•g⁻¹, respectively. Additionally, as compared with samples dried in the shade, the contents of dihydrotanshinone, cryptotanshinone and tanshinone Ⅰ were increased 2.9% (P>0.05), 45.3% (P<0.05) and 34.5% (P<0.05), respectively; however, the content of tanshinone ⅡA was decreased by 44.1% (P<0.05). The water-soluble active components (rosmarinic acid and salvianolic acid B) of roots of S. miltiorrhiza f. alba, had the highest contents when the samples were first dried at 30 ℃ for six hours and then 70 ℃ for three hours, and the contents were 3.83,55.44 mg•g⁻¹, increased by 62.3% (P<0.05) and 109.1% (P<0.05) respectively as compared with the samples dried in the shade. Variable temperature drying can significantly affect the contents of active components in roots of S. miltiorrhiza f. alba. As compared with the traditional process of shade-drying process, low temperature drying can significantly increase the content of water-soluble active components and also with significant promotion effect on the liposoluble components such as tanshinone ⅡA, cryptotanshinone and tanshinone Ⅰ. The variable temperature drying mode, can effectively shorten the process of drying and provide theoretical basis for industrial processing of roots of S. miltiorrhiza f. alba.

Survival of a newborn from a pregnant woman with rabies infection

Rabies is very common in Peoples Republic of China. Each year thousands of people die because of this disease, but rabies diagnosed in pregnancy is very rare. Case Presentation In this study, we report the case of a pregnant woman who was infected with the rabies virus after a dog bite. The symptoms of rabies appeared in labor and she died after pregnancy. Her baby and husband did not develop the disease. Conclusion The phenomenon that the newborn infant was healthy may be related to the protective role of placenta in resisting the invasion of the rabies virus or the absence of systemic viremia. The prompt administration of vaccines and anti-rabies immunoglobulin to the infant may have also contributed to his survival.

Survival of a newborn from a pregnant woman with rabies infection

Background Rabies is very common in People's Republic of China. Each year thousands of people die because of this disease, but rabies diagnosed in pregnancy is very rare. Case Presentation In this study, we report the case of a pregnant woman who was infected with the rabies virus after a dog bite. The symptoms of rabies appeared in labor and she died after pregnancy. Her baby and husband did not develop the disease. Conclusion The phenomenon that the newborn infant was healthy may be related to the protective role of placenta in resisting the invasion of the rabies virus or the absence of systemic viremia. The prompt administration of vaccines and anti-rabies immunoglobulin to the infant may have also contributed to his survival.(AU)

The experimental study of double-bridging facial nerve defect in rabbits by chitosan-collagen chambers / 华西口腔医学杂志

<p><b>OBJECTIVE</b>To evaluate the feasibility of repairing the long defects in rabbits by double-bridging technique and chitosan-collagen chambers.</p><p><b>METHODS</b>Thirty Chinese rabbits were divided into three groups randomly. 15 mm defects in right facial nerve of rabbits were repaired in three groups: double-bridging (group I ), nerve autografts (group II), and normal control (group III). General observation, electrophysiological study, histological study and image analysis were performed 12 weeks postoperatively. All results were used to evaluate the nerve regeneration.</p><p><b>RESULTS</b>Chitosan-collagen chambers were obviously degraded 12 weeks postoperatively and there was no foreign body reaction at this stage. They also restrain the formation of neuroma and provided a good microcircumambience for nerve regeneration. The recovery of nerve regeneration were good in group I. The nerve conduction velocity (NCV) and compound muscle action potential (CMAP) of regenerated nerve were analyzed, which showed that there was no significant difference between the group I and group II (P > 0.05), but they all did not recover to normal level(P < 0.01).</p><p><b>CONCLUSION</b>Double-bridging technique is a simple method for treatment of long nerve defect and its effect is certain. Chitosan-collage chambers is suitable to implant into body in order to repair a nerve defect with excellent biocompatibility.</p>