Résumé
<p><b>AIM</b>To introduce the manufacture and use of a simple, new type of drop recorder of frog heart.</p><p><b>METHODS</b>To improve the perfusion device of (see text for symbol) and Straub method. Two electrodes of drop recorder were fixed in an injector of 20 ml. The input tube, output tube and resistance tube were all made of plastic material.</p><p><b>RESULTS</b>This device could be used to observe effects of preload, after-load, hormone and electrolyte on the cardiac output in isolated frog heart.</p><p><b>CONCLUSION</b>The new type of drop recorder was economical and could be easily operated, it could be also connected to computer. Using the new type of drop recorder, effects of various physical and chemical factors on cardiac function could be observed directly, accurately.</p>
Sujets)
Animaux , Électrodes , Coeur , Physiologie , Pompes à perfusion , Ranidae , Poids et mesuresRésumé
The cardiac electrophysiological effects of genistein (GST) were examined in guinea pig papillary muscle using intracellular microelectrode technique. The results obtained are as follows. (1) Duration of action potential (APD) in normal papillary muscles was decreased by GST (10 100 micromol/L) in a concentration-dependent manner. (2) In partially depolarized papillary muscles, 50 micromol/L GST not only reduced APD, but also decreased the amplitude of action potential, overshoot and maximal velocity of phase 0 depolarization. (3) Pretreatment with N( )-nitro-L-arginine (L-NNA, 5 mmol/L) failed to affect the above effects of GST (50 micromol/L)on papillary muscles. (4) 17beta-estradiol (E(2), 5 micromol/L) or GST (10 micromol/L) alone did not affect action potential, while GST combined with E(2) at the same doses shortened APD significantly. All these results indicate that the effects of GST on papillary muscles are likely due to a decrease of calcium inflow which is not mediated by NO and that GST has a facilitative or synergetic action with E(2).
Sujets)
Animaux , Femelle , Mâle , Potentiels d'action , Agmatine , Pharmacologie , Relation dose-effet des médicaments , Synergie des médicaments , Électrophysiologie , Oestradiol , Pharmacologie , Oestrogènes nonstéroïdiens , Pharmacologie , Génistéine , Pharmacologie , Cochons d'Inde , Isoflavones , Muscles papillaires , Physiologie , Phyto-oestrogènes , Préparations à base de plantesRésumé
<p><b>AIM</b>To study the effects of the 50 Hz-filter circuit in a microelectrode amplifier on cardiac action potential waveform and parameters.</p><p><b>METHODS</b>Cardiac action potential signals were fed into a microcomputer through a glass microelectrode, a microelectrode amplifier, a differentiator and A/D converter. The cardiac action potential signals were recorded and analyzed with 50 Hz-filter circuit and without it, and the frequency spectrum in the signals was analyzed with the fast Fourier transformation.</p><p><b>RESULTS</b>When the 50 Hz-filter circuit was used, the phase 0 of the potential waveform was seriously distorted and prolonged. The maximal rate of depolarization at the phase 0 was cut down, while the other parameters were not effected.</p><p><b>CONCLUSION</b>There has already been much 50 Hz element in the action potential waveform. During amplifying the cardiac action potential signal, the 50 Hz-filter circuit should not be turned on. Otherwise, the experiment results will be effected.</p>