The early diagnostic and treatment value of combined detection of hemolysis three tests and measurement of serum total bilirubin for hemolytic disease of newborn / 中国基层医药

Objective To explore the early diagnostic and treatment value of combined detection of direct antiglobulin test ,free antibody and antibody releasing test ( hemolysis three tests ) and measurement of serum total bili-rubin for hemolytic disease of newborn ( HDN) .Methods Hemolysis three tests were carried out with micro column gel technology in 212 blood samples from neonatal jaundice ( derived from the O type mothers ) and positive rate differ-ences in hemolysis three tests were compared .Patients were divided into 4 groups by the antibody results in hemolysis three tests:group 1[direct antiglobulin test ( +),free antibody test ( +) and antibody releasing test ( +)],group 2[(direct antiglobulin test(+),free antibody test(-) and antibody releasing test(+)],group 3[direct antiglobu-lin test(-),free antibody test(+) and antibody releasing test(+),and group 4[direct antiglobulin test( -),free antibody test (-) and antibody releasing test (+) ] .Constituent ratio was compared .The total bilirubin in serum from four groups were determined by automatic biochemical analyzer and compared .Results The positive rates of direct antiglobulin test,free antibody test and antibody release test were 18.4%(39/212),33.5%(71/212) and 39.6%(84/212) respectively.The positive rate had no significant difference between antibody release test and free antibody test(χ2 =1.70,P>0.05),but compared with direct antiglobulin test ,the differences were significant (χ2 =23.18, 12.58,all P0.05).Conclusion Combined detection of serum total bilirubin and serum hemolysis three tests can be used as the early diagnosis and patients condition of HDN ,and contribute to the early control of neo-natal hemolytic increase ,reduce complications and sequelae .

Preliminary study on the biological functions of interferon-λ in human esophageal carcinoma cells / 中华微生物学和免疫学杂志

Objective To investigate the biological function of IFN-λ in 7 human esophageal carcinoma cells. MethodsThe gene expression of IL-28α, IL-10β and antiviral molecule was examined with PCR. The MHC molecules expression and the profiles of cell cycle were analyzed with flow cytometer. Cell proliferation was evaluated with MTT assay. ResultsAll of esophageal carcinoma cells express the gene of II-28α and IL-10β. IFN-λ induced or augmented the gene expression of antiviral molecules, 2′5′-OAS and MxA. IFN-λ enhanced the MHC class Ⅰ molecule expression. IFN-λ inhibited the growth of esophageal carcinoma cells through the regulation of cell cycle distribution. ConclusionEsophageal carcinoma cells express the IFN-λ receptor complex. IFN-λ has the antiviral, anti-proliferative and immunoregulation activity.