RÉSUMÉ
Objective To establish a method for the determination of gastrodin in Tianyuan Granula. Methods The sample was refluxed with methanol, then eluted with 80 % ethanol through the alumina column(neutral). The chromato-graphic conditions were as follows: Diamonsil Cts chromatographic column(250 mm ×4.6 mm, 5 μm) with a mobile phase of acetonitrile-0. 15 % phosphoric acid (1 : 99), the detection wavelength being at 220 nm and the flow rate be-good (r=0.9999 95, n=7).The average recovery was 99.09 % and RSD=1. 50 % (n=6). Conclusion The method for determination of gastrodin in Tianyuan Granula is accurate, and with a good reproducibility.
RÉSUMÉ
Objective To establish a method for the determination of Puerarin in Huanglian Jiangtang Tablets. Methods The sample was extracted with 30 %ethanol. The chromatographic conditions were as follows:Diamonsil C18 chromatographic column(250 mm?4.6 mm,5?m)with a mobile phase of acetonitrile-0.5 %glacial acetic acid,the detection wavelength being at 250 nm and the flow rate being 1.0 mL?min-1. Results A linearity was obtained from 0.338 ?g to 2.336 ?g of Puerarin in Huanglian Jiangtang Tablets with a good correlation (r=0.999997,n=7).The average recovery was 100.2% and RSD=1.80%(n=6). Conclusion This method for determination of Puerarin in Huanglian Jiangtang Tablets is easy,sensitive,specific and accurate.
RÉSUMÉ
Objective To establish a method for the determination of berberine hydrochloride in Huanglian Jiangtang Pill. Methods The sample was extracted with hydrochloric acid-methanol(1 ∶ 100). The chromatographic conditions were as follows:Diamonsil C18 chromatographic column(250 mm? 4.6 mm,5 ? m)with a mobile phase of acetonitrile-0.05 mol/L potassium dihydrogen phosphate(28 ∶ 72),the detection wavelength at 350 nm and the flow rate being 1.0 mL? min-1. Results A good linearity was obtained from 0.244 ? g to 2.928 ? g of berberine hydrochloride in Huanglian Jiangtang Pill(r=0.999 9,n=7).The average recovery was 99.07 % and RSD=1.30 %(n=6). Conclusion This method is simple,sensitive,specific and accurate for determination of berberine hydrochloride in Huanglian Jiangtang Pill.
RÉSUMÉ
AIM: To establish a RP-HPLC method for determining ephedrina hydrochloridum and pseudoephedrine hydrochloride in Chuanliting Spray(Herba Ephedrae,Folium Artenisiae argyi,Radix et Rhizoma Asari,etc.). METHODS: HPLC conditions were as follows: C_(18)(4.6 mm?250mm,5?m) column,0.2% phosphoric acid—acetonitrile(96∶4) as mobile phase with flow rate of 1mL/min,and the detection wavelength at 205nm.(RESULTS:) The calibration curve of ephedrina hydrochloridum was linear between 0.448-3.416?g(r=(0.999 8,) n=7).The average recovery was 100.26% and RSD=2.17%(n=5).The calibration curve of pseudoephedrine hydrochloride was linear between 0.16-1.12?g(r= 0.999 1,n=7).The average recovery was 101.60% and RSD =2.19%(n=5). CONCLUSION: The method is convenient and efficient,and can be used for quantitative analysis and quality control of this preparation.