Résumé
Objective: To observe the preventive effect of Bu Shen Jian Pi Capsule on age-related cochlear hair cells apoptosis in C57 BL /6J mice,and research its mechanism of action.Methods: 30 new born C57 BL /6J mice were divided into 2 groups,with 10 animals in control group(CG) and 20 animals in Bu Shen Jian Pi Capsule (experimental group,EG).The animals in CG were fed regularly,while those in EG were fed regularly at the first one months after the birth and then,commonly fed water was substituted by the medical solution of Bu Shen Jian Pi Capsule for these animals in EG.The experimental course lasted for seven months.The cochleas of the two groups of mice were taken out for cochlear surface preparation,then being observed under optical microscope with a comparative analysis on the status of degenerative changes in the hair cells of cochlear between the tow groups.Results: Cochlear hair cells damage of CG animals showed aggravating tendency with the increase of the age,and followed the developmental rules of lesion progression in cochlear hair cells from the basal turn to the apical turn and with the number of outer hair cells loss greater than that of inner hair cells.In contrast,the cochlear damage of animals of EG was relatively milder than that in animals of CG,compared among the animals at the same age between them.Conclusion: This study showed that Bu Shen Jian Pi Capsule could postpone age-related cochlear hair cells apoptosis in C57 BL /6J mice,therefore,it suggested that Bu Shen Jian Pi Capsule might possess the pharmacological action of preventing presbyacusis.We assume the possible protective mechanism of Bu Shen Jian Pi Capsule might be related to increase of cells cAMP,regulation of metabolism of nucleic acid,and protectoin or stabilization of DNA structure.