Résumé
A monoclonal antibody (8H5), which showed strong neutralization activity against 33 strains of H5N1 viruses isolated from hosts at various regions from 2002 to 2006, was characterized in our lab recently. This result indicated the presence of highly conserved neutralizing site on hemagglutinin (HA) of various H5N1 subtypes. In the present study, the peptide phage display technique was applied to generate mimotope of the conserved neutralizing epitope recognized by 8H5 mAb. Five peptides displayed on phage were identified to specifically bind to 8H5 mAb. One of the five peptides, 123, was further displayed on the virus-like particle assembled from aa 1-149 fragment of HBcAg. The chimeric particle HBc-T123 conserved the specific binding to 8H5 mAb, and competed with H5N1 viruses for 8H5 mAb. The antiserum induced by HBc-T123 intensively stained on SF21 cells infected by recombinant baculovirus containing HA gene of YU22 virus, indicating the production of cross-reactive antibody to H5N1 HA.
Sujets)
Animaux , Humains , Souris , Séquence d'acides aminés , Anticorps monoclonaux , Allergie et immunologie , Épitopes , Chimie , Génétique , Allergie et immunologie , Glycoprotéine hémagglutinine du virus influenza , Chimie , Génétique , Allergie et immunologie , Sous-type H5N1 du virus de la grippe A , Chimie , Génétique , Allergie et immunologie , Grippe humaine , Virologie , Souris de lignée BALB C , Données de séquences moléculaires , Banque de peptidesRésumé
0.05),12 h(96.33?76.09),24 h(98.63?75.73),48 h(100.77?69.48) post-CPB were significantly lower than that of pre-CPB(125.53?70.85)(t=2.316,2.139,2.058 Pa0.05).Conclusions The early damage of lung function after CPB is obviously in infants.Accurate management of circulation and respiration are important for reducing acute lung injury and improving pulmonary function.