Your browser doesn't support javascript.
loading
Montrer: 20 | 50 | 100
Résultats 1 - 2 de 2
Filtre
1.
Chinese Journal of Biotechnology ; (12): 887-898, 2012.
Article Dans Chinois | WPRIM | ID: wpr-342432

Résumé

To develop a specific, rapid, and convenient immunochromatography assay (ICA) to detect melamine residues in dairy products and feed sample. Colloidal gold particles labeled with purified monoclonal antibody against anti-melamine were used as the detector reagent. The MEL-OVA (the conjugate of melamine and ovalbumin) and goat anti-mouse melamine IgG were blotted on the test and control regions of nitrocellulose membrane. The strip was then assembled with sample pad, absorbing pad, and dorsal shield. The limit of detection (LOD) is 50 microg/L. The test trip was applied to detect melamine in milk, milk powder, and animal feeds, with detection limits of 100 microg/L for milk, 100 ng/g for milk powder, 200 ng/g for feeds. Compared to LC-MS/MS, the ICA could be used to screen a large number of dairy products and feed samples for melamine residue.


Sujets)
Animaux , Bovins , Anticorps monoclonaux , Chimie , Produits laitiers , Contamination des aliments , Or colloïdal , Chimie , Chromatographie d'affinité , Méthodes , Lait , Chimie , Bandelettes réactives , Chimie , Sensibilité et spécificité , Triazines
2.
Chinese Journal of Biotechnology ; (12): 418-423, 2009.
Article Dans Chinois | WPRIM | ID: wpr-286695

Résumé

Sorghum (Sorghum bicolor L.) was one of the most important crops in the world next to wheat, rice, maize, soybean and barley. Using the callus derived from immature inflorescence as the recipients, we efficiently transformed sorghum varieties 115, ICS21B and 5-27 with the insecticidal Bacillus thuringiensis (Bt) cry1Ab gene carried in the T-DNA of binary vectors which contained hygromycin resistance gene and gus gene via Agrobacterium tumefaciens. After gradient selection with hygromycin, a total of 21 independent transgenic plant lines, 52 transgenic plants were regenerated, and the average stably transformation efficiency was 1.9%. The integration and transcription of cry1Ab gene in transgenic sorghum was confirmed by PCR analysis, Southern blotting and RT-PCR analysis. The Bt proteins were expressed in most transgenic plants with different level from plant to plant by Western blotting and ELISA assay. According to insect bioassay in laboratory, the transgenic plants with a relatively high level of Bt gene expression displayed insect-resistance to pink rice borer (Sesamina inferens).


Sujets)
Agrobacterium tumefaciens , Génétique , Métabolisme , Protéines bactériennes , Génétique , Métabolisme , Endotoxines , Génétique , Métabolisme , Hémolysines , Génétique , Métabolisme , Lutte biologique contre les nuisibles , Végétaux génétiquement modifiés , Génétique , Sorghum , Génétique , Transformation génétique
SÉLECTION CITATIONS
Détails de la recherche