Stability indicating RP-HPLC method for simultaneous determination of gatifloxacin and dexamethasone in binary combination

Abstract In this study, conditions were optimized for development of a simple RP-HPLC method for simultaneous analysis of gatifloxacin and dexamethasone in different matrices like pharmaceuticals, human serum and urine. Good separation of gatifloxacin and dexamethasone from the induced degradation products was accomplished using C8 as stationary phase; 0.02 M phosphate buffer (pH 3.0) and methanol (42:58 v/v) as mobile phase. The concentration was measured with DAD at 270 nm. Linearity was observed in the range of 0.000040-0.000280 mol/L for gatifloxacin (r2≥0.999) and 0.000013-0.000091 mol/L for dexamethasone (r2≥0.999). Both the analyte peaks were completely separated from the peaks of induced degradation products as indicated by the peak purity index (≥0.9999 for both analytes). The optimized method is recommended to be used for concurrent analysis of gatifloxacin and dexamethasone in different matrices.

Compositional studies and Biological activities of Perovskia abrotanoides Kar. oils

BACKGROUND: Current study has been designed to evaluate the chemical composition of essential and fixed oils from stem and leaves of Perovskia abrotanoides and antioxidant and antimicrobial activities of these oils. RESULTS: GC-MS analysis of essential oil identified 19 compounds with (E)-9-dodecenal being the major component in stem and hexadecanoic acid in leaves. In contrast, GC-MS analysis of fixed oil showed 40 constituents with α-amyrin the major component in stem and α-copaene in leaves. The antioxidant activity showed the highest value of 76.7% in essential oil from leaves in comparison with fixed oil from stem (45.9%) through inhibition of peroxidation in linoleic acid system. The antimicrobial assay tested on different microorganisms (e.g. E. coli, S. aureus, B. cereus, Nitrospira, S. epidermis, A. niger, A. flavus and C. albicans) showed the higher inhibition zone at essential oil from leaves (15.2 mm on B. cereus) as compared to fixed oil from stem (8.34 mm onS. aureus) and leaves (11.2 mm on S. aureus). CONCLUSIONS: The present study revealed the fact that essential oil analyzed from Perovskia abrotanoides stem and leaves could be a promising source of natural products with potential antioxidant and antimicrobial activities, as compared to fixed oil.

In Vitro Antimicrobial and Haemolytic Studies of Bambusa arundinaceae leaves.

Bambusa arundinacea locally known as Bans or bamboo, a perennial fastest growing plant. This plant is medicinally much famous. The biological activities of this plant were carried out by using standard procedures. The aim of this work was to investigate the in vitro antimicrobial and haemolytic activities of Bambusa arundinaceae leaves. The GC-MS analysis revealed that the major components determined in n-hexane extract were as n-Nonane (17.14%), 2,2,3-Trimethylbutane (5.85%), Tridecane (5.42%), and 3,4,5,6-Tetramethyloctane (5.32%). The disc diffusion and modified resazurin microtitre-plate assays were used to evaluate the inhibition zones (IZ) and minimum inhibitory concentration (MIC) of B. arundinaceae leaves extracts. The plant extracts showed considerable antimicrobial activity. The heamolytic activity against human blood erythrocytes (RBCs) was studied and the % lysis of RBCs was found to be in the range of 1.03 to 4.81. The results of the present study confirmed the considerable antimicrobial and haemolytic activities of the plant Bambusa arundinaceae.