Résumé
Objective To investigate the correlation between the levels of serum tumor necrosis factor-α(TNF-α),glial fibrillary acidic protein(GFAP) and the malignant degree and prognosis of patients with brain glioma.Methods A total of 90 patients with brain glioma were collected from March 2013 to September 2015 in Shaanxi Provincial People's Hospital.The patients were divided into low malignant degree group (n =43) and high malignant degree group (n =47) according to the pathological grade of malignant degree.Fifty-two healthy volunteers were selected as control group at the same period.The fasting venous blood 5 mL of the patients with brain glioma was collected at the time points of pretherapy,the 14th day after operation,the first day after radiotherapy and chemotherapy.The fasting venous blood 5 mL was collected on the day of physical examination in the control group.The level of serum TNF-α was detected by enzyme-linked immunosorbent assay,and the level of serum GFAP was measured by double antibody sandwich method.Results The levels of serum TNF-α and GFAP in the low malignant degree group and high malignant degree group were significantly higher than those in the control group (P ＜ 0.05),and the levels of serum TNF-α and GFAP in the high malignant degree group were significantly lower than those in the low malignant degree group before treatment (P ＜ 0.05).The levels of serum TNF-α and GFAP at the time points on the 14th day after operation and after radiotherapy and chemotherapy were significantly lower than those before treatment (P ＜ 0.05);the levels of serum TNF-α and GFAP after radiotherapy and chemotherapy were significantly lower than those on the 14th day after operation in the low malignant degree group (P ＜ 0.05).The level of serum TNF-α on the 14th day after operation was significantly lower than that before treatment in the high malignant degree group (P ＜ 0.05).There was no significant difference in serum GFAP level between the 14th day after operation and before treatment in the high malignant degree group (P ＞ 0.05).The levels of serum TNF-α and GFAP after radiotherapy and chemotherapy were significantly lower than those before treatment and the 14th day after operation in the high malignant degree group (P ＜ 0.05).The levels of serum TNF-α and GFAP in the patients with complete remission were significantly lower than those in the patients with partial remission,stable disease and progressive disease after treatment (P ＜ 0.05).The levels of serum TNF-α and GFAP in the patients with partial remission were significantly lower than those in the patients with stable disease and progressive disease after treatment (P ＜ 0.05).The levels of serum TNF-α and GFAP in the patients with stable disease were significantly lower than those in the patients with progressive disease after treatment (P ＜ 0.05).Conclusions The levels of serum TNF-α and GFAP are correlated with the malignant degree and the therapeutic effect in patients with brain glioma.It can be used as a biological marker to evaluate the malignant degree,therapeutic effect and prognosis of brain glioma.