Résumé
Objective:To investigate the effect of X-box binding protein 1(XBP1) on the apoptosis of H9C2 cardiomyocytes under high glucose by XBP1/CHOP/Puma signaling pathway.Methods: The experiment were divided into four groups:low glucose group (LG group),high glucose group (HG group),high glucose+siRNA-NC group (HG+NC group),high glucose+siRNA-XBP1 (HG +siRNA).The expression of XBP1,C/EBP homologous protein (CHOP),p53 upregulated modulator of apoptosis(Puma), Caspase-3 in the cells after transfected were detected by Western blot;CCK-8 were used to detect the cell proliferation.Cell apoptosis was detected by flow cytometry.Results:siRNA-XBP1 reduced the expression of XBP1 in cardiomyocytes(P<0.05).Compared with LG group,the survival rate of HG group,HG +NC group and HG +siRNA group was significantly decreased (P<0.05),and the apoptosis rate was significantly increased(P<0.05).Compared with HG group,the survival rate of HG+siRNA group was significantly increased (P<0.05),and the apoptosis rate was significantly decreased(P<0.05).XBP1,CHOP,Puma in HG group,HG +NC group and HG+siRNA group was significantly higher than in LG group(P<0.05).Compared with HG group,the expression of XBP1,CHOP, Puma and Caspase-3 in HG+siRNA group was significantly lower(P<0.05).Conclusion:XBP1 inhibits cardiomyocyte apoptosis and promotes proliferation by regulating XBP1/CHOP/Puma signaling pathway.
Résumé
Objective:To describe long-term results of locking plate used for the treatment of non-osteoporotic fresh three-and four-part proximal humeral fractures with at least 2 years follow-up.Methods:The functional outcomes and the complications of non-osteoporotic three-and four-part fresh proximal humeral fractures treated with locking plate were assessed retrospectively.The active range of motion,the Constant score,the University of California at Los Angeles (UCLA) shoulder score,the visual analogue score (VAS) were employed to evaluate the postoperative shoulder function,and the radiographic images were taken to evaluate the neck-shaft angle of the proximal humeral and postoperative implant-related complications.Results:From January 2007 to October 2014,107 consecutive fresh three-and four-part non-osteoporotic fresh proximal humeral fractures were treated with a locking plate in our department.Among them,67 patients completed at least 2 years follow-up.The average follow-up time was (43.9 ± 23.3) months (range:24-108 months).The mean Constant score was 87.1 ± 11.7 (range:51-100),the mean UCLA score was 30.5 ± 3.9 (range:18-35),the mean VAS score was 1 ±2 (range:0-7).The mean active forward flexion was 159.0° ± 19.3° (range:80°-180°),the mean external rotation was 36.8°± 19.5° (0°-80°) and the mean internal rotation was T11 level (T2-LS level).There were 11 patients who suffered from complications.Screw perforations were observed in 5 (7.5％) patients,avascular necrosis of the humeral head was observed in 9 (13.4％) patients and traumatic osteoarthritis was observed in 5 (7.5 ％) patients.Six patients showed two or more complications.There was no significant difference in outcomes when comparing the patients with three-part fractures (31 patients) with those with four-part fractures (36 patients).The rates of complications and avascular necrosis were significantly higher in the four-part fracture group than in the three-part fracture group.Conclusion:The locking plate is an effective method in treating three-and four-part non-osteoporotic fresh proximal humeral fractures.Strict surgical indication and precise surgical skill are the key points for successful treating non-osteoporotic fresh proximal humeral fractures.There is a higher rate of complications and avascular necrosis of the humeral head in the four-part fractures than in the three-part fractures.