Résumé
Two compounds were isolated from 95% ethanol extract of the gum resin of Boswellia carterii by silica gel column chromatography (CC) and high-performance liquid chromatography (HPLC). Their structures were identified with IR, UV, NMR and HR-ESI-MS spectroscopic data as 7α-hydroxy-3,11-dioxo- tirucalla-8,24-dien-21-oic acid (1) and 21β-hydroxy-3-acetyl-11-keto-β-boswellic acid (2). In addition, their absolute configurations were also identified by ECD calculations. Among them, compound 1 is a new compound and the absolute configuration of compound 2 is confirmed by ECD calculation for the first time.
Résumé
Objective : To develop a quantitative method of 7α-hydroxy-4-cholesten-3-one (C4), cholic acid (CA) and chenodeoxycholic acid (CDCA) in human plasma. Methods ¡¤ After extraction of C4, CA and CDCA with acetonitrile from plasma, they were quantified with stand-ard curve corrected by the internal standards based on Ultra-high performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS). Results ¡¤ The limits of detection (LOD) of C4, CA and CDCA were 0.16, 0.02 and 0.04 nmol/L respectively; All three metabolites had good linear relationships (correlation coefficients R2 were over than 0.998). The relative standard deviations (RSDs) of repeatabilities were below 3.0%. The RSDs of inter-day and intra-day precision were less than 6%, and the RSDs of stabilities at 4 °C were below 10% within 7 days. The average added recoveries of C4, CA and CDCA were 97.7%, 113.3% and 105.0%, respectively. Conclusion ¡¤ This method is of high detective sensi-tivity, good precision and stability, which meets the quantitative requirements of plasma biological samples.
Résumé
OBJECTIVE: To study the chemical constituents of Catharanthus roseus. METHODS: Various column chromatograghic methods on silica gel, Rp-18, and Sephadex LH-20 were applied for the isolation and purification of the 95% ethanol extract. The structures were elucidated by their physicochemical properties and spectral data. RESULTS: Twenty-two compounds were obtained and identified as ursolic acid (1), daucesterol (2), tetrahydroalstonine (3), 7α-hydroxy-β-sitosterol (4), vindoline (5), β-sitosterol (6), aurantiamide acetate (7), lochnericine (8), oleanolic acid (9), ajmalicine (10), (22E, 24R)-ergosta-7, 22-dien-3β, 5α, 6β-triol (11), betulinic acid (12), stigmasterol (13), quercetin (14), keampferol (15), vindorosine (16), catharanthine (17), diaaurantiamide acetate (18), reserpine (19), panarine (20), serpentine (21), and 16R-E-isositsirikine (22). CONCLUSION: Compounds 4, 7, 11, 13, 18, and 20 are isolated from C. roseus for the first time.
Résumé
To study the chemical constituents from the stems of Cipadessa cinerascens. The constituents were separated by column chromatography and their structures were elucidated by spectral data analyses. Seventeen chemical constituents were isolated from the EtOAc extract in the stems of C. cinerascens and their structures were identified as lanost-7-en-3-one-22, 25-epoxy-23, 24-acetone acetal (1), chisopanin M (2), 3β-hydroxy-5-en-stigmast (3), 7α-hydroxy-4-en-3-one-stigmast (4), 3β-hydroxy-5-en-7-one-stigmast (5), 7α-hydroxysitosterol (6), 22E-7α-methoxy-5α, 6α-epoxy-8 (14), 22-dien-3β-hydroxy-ergosta (7), 7β-hydroxy-4-en-3-one-cholest (8), 3β-acetyloxy-2β, 4β-dihydroxy-16-one-pregnan (9), 17α, 20R-dihydroxy-3, 16-dione-pregnan (10), 2β, 3β-dihydroxy-16-one-pregnan (11), 3β, 7α-dihydroxy-20-one-pregnan (12), 2α, 3β-dihydroxy-5-en-20-one-pregnan (13), 1, 4-dien-3, 16-dione-2-hydroxyandrosta (14), 5-en-17-one-3β, 16β-dihydroxyandrost (15), apegenin (16), and annuionone D (17). Conclusion All the compounds except 14 and 17 are obtained from this plant for the first time.