Platelet Distribution Width from Two Automated Hematology Analysers: A Correlation Analysis

Platelet distribution width (PDW) is an index for platelet size variation. In this study, we analysed the correlation between PDW values obtained using two different hematology analysers that employ different measurement methods. Complete blood cell parameters including PDW for 153 healthy individuals were measured using both, ADVIA 2120i (Simens AG, Germany) and XN-3000 (Sysmex, Japan). The PDW values measured using the two hematology analysers showed a moderate correlation (r=0.661, P0.900, P<0.001). PDW obtained using XN-3000 showed a strong correlation with mean platelet volume, whereas PDW obtained using ADVIA 2120i did not. The reference values in this group were 40.0%–64.2% in ADVIA 2120i and 9.0–16.0 fL in XN-3000. In conclusion, PDW values obtained using ADVIA 2120i and XN-3000 are not interchangeable. In laboratories equipped with more than one hematology analyser, a particular analyser should be used consistently for monitoring a particular patient.

Evaluation of the Measurement of Nucleated Red Blood Cells in Neonatal Blood with ADVIA 2120 / 임상검사와정도관리

BACKGROUND: Nucleated red blood cells (NRBCs) are immature erythrocytes. The current line of thought is that increased NRBC production in the immediate neonatal state primarily reflects hypoxic injury and a direct response to mediators of inflammation in newborns with early-onset neonatal sepsis. So, the accurate, reproducible, and timely reporting of NRBC is an important function of the clinical laboratory. The aim of this study is to evaluate the performances of the ADVIA 2120 Hematology System (Siemens, USA) in the NRBCs measurement and compare them with the conventional microscopic method. METHODS: The study was performed in Kangnam Sacred Heart Hospital from March to September, 2009. One hundred twenty neonatal samples were submitted to measure NRBC count. For comparison, 225 blood samples except neonates were also included. The specimens were analyzed for the NRBC counts both with ADVIA 2120 analyzer and by microscopic method. RESULTS: Correlations of NRBC counts form the ADVIA 2120 compared with microscopic results were excellent in neonatal and in all specimens, r=0.8630 and r=0.9012, respectively. Sensitivity and specificity for the presence of NRBC was 98.2% and 94.2%, respectively. Concordance rate was 95.5%. There were some discordant results in cases of chronic lymphocytic leukemia and iron deficient anemia. CONCLUSIONS: The ADVIA 2120 NRBC method correlated well with the manual microscopic method. The automatic correction of the WBC count and differential, the shorter turnaround time compared to microscopic counting are additional advantages of automatic method. The automatic NRBC counting is considered to be a useful method in neonatal hematology.