Association of the lipoprotein lipase and Apolipoprotein C-II gene polymorphisms with risk of dyslipidemia in smokers and non-smokers male

Objective: Dyslipidaemia is considered a metabolic abnormality andan important risk factor that leads to atherogenic cardiovascular diseases. Cigarette smoking is associated with dyslipidaemia. This study aimed to demonstrate whether lipoprotein lipase enzyme (LPL) and Apolipoprotein CII (APOCII) gene polymorphisms can be considered as independent genetic risk factors for dyslipidaemia among smokers with various smoking durations. Methods: A total of 185 males (90 smokers and 95 non-smokers)were included in this study, Lipid profiles were measured and DNA was isolated. The LPL-Hind III and APO CII-Ava II polymorphisms were determined using the polymerase reaction-restriction fragment length polymorphisms (RFLP) technique. Results: For the LPL-Hind IIIpolymorphism HþHþ genotype group, the triglycerides TG and very-lowdensity lipoprotein cholesterol VLDL-C concentrations were significantly higher and the high-density lipoprotein cholesterol HDL-C concentration was significantly lower than those of the HeH- genotype. ForAPO CII-Ava II polymorphisms, compared with those of the A2A2 genotype group, the total cholesterol TC, TG, low-density lipoprotein cholesterol LDL-C and VLDL-C concentrations were significantly increased in the A1A2 genotype group, while the HDL-C concentration was significantly decreased. Conclusions: The study revealed that the HþHþ or H þ H-genotype of the LPL-Hind III polymorphism and the A1A1or A1A2 genotype of the APOCII-Ava II polymorphism were at higher risk of developing dyslipidaemia compared to the HeH- genotype of the LPL-Hind III polymorphism and A2A2 genotype of the APOCII-Ava II polymorphism.

Genetic Analyses Of Plasma ApoCII By Two-Dimensional Gel Electrophresis In Two Population Groups Of Panjab.

In order to study genetic variability of apolipoprotein C-II in Ramgarhias and Ramdasias, two-dimensional gel electrophoresis with the combination of isoelectric focusing (IEF) and density gradient SDS-polyacrylamide gel electrophoresis (DG-SDS-PAGE) have been employed. ApoC-II exhibits three common polymorphic alleles CII-1, CII-2 and CII-3 with pooled frequencies of 0.883, 0.110 and 0.007 respectively. The rare allele CII-4 is absent in these two groups. Mean heterozygosity is found to be 0.208+ 0.024 at apoC-II structural locus. The frequency of apoCII-2 in Punjab is observed to be the highest in the world except Ukainers.