Effect of paeonol on adip/camkk(i/ampk pathway in alcoholic fatty liver in mice / 中国药理学通报

Aim To investigate whether the Adip/CaMKKp/AMPK pathway is involved in the treatment of alcoholic fatty liver in mice treated with paeonol. Methods After administration of paeonol on the basis of the mouse alcoholic fatty liver model, the inflammatory factors and TG and TC were detected by Elisa method to verify the validity of the model and drug treatment; meanwhile, the expression levels of Adip and ACC were detected by Elisa, and the nucleic acid and protein levels of Adip, CaMKK{3 and AMPKaby PCR and WB. Results Adip expression levels did not change significantly in each treatment group. Compared with control group, as for the expression of CaMKKp and AMPKa at the nucleic acid and protein-phosphorylation levels, ihe ElOH group was down-regulated to varying degrees, and the Sil and Pae groups improved the phenomenon to varying degrees. Compared with control group, as for the expression of SREBPlc at the nucleic acid and protein levels, the EtOH group was up-regulated to varying degrees, and the Sil and Pae groups improved the phenomenon to varying degrees. Conclusions The changes of theAdip/CaMKKp/AMPK signaling pathway confirm that pae-onol reduces steatosis injury of alcoholic fatty liver and inflammatory level in mice via this pathway.

Regulation of the proliferation and differentiation of mice bone marrow mesenchymal stem cells by CKIP-1 in vitro / 实用口腔医学杂志

Objective:To investigate the regulation of proliferation and differentiation of bone marrow mesenchymal stem cells(BM-SCs)by CKIP-1 in vitro.Methods:BMSCs from CKIP-1 nock out(KO)and wild type(WT)C57 mice were isolated and cultured u-sing adherence method in vitro.BMSCs of the 3rd passage were induced to osteogenic and adipgenic differentiation.Cell proliferation was examined by MTT assay.Cell surface markers were tested by FCM.The osteogenic and adipogenic differentiation was studied by alkaline phosphatase (ALP)staining,alizarin red staining and oil red O staining.Results:The proliferation and cell marke expression of the 2 groups were similar.ALP staining of KO group was strong than that of WT group after osteogenic induction.Alizarin red stai-ning showed that there were more mineralized nodules in BMSCs of KO group than in those of WT group.Oil red O staining of KO mice BMSCs was stronger than that of WT.Conclusion:CKIP-1 deficiency can enhance the osteogenic and adipogenic differentiation without influence on the proliferation of BMSCs.