Résumé
Alocasia indica is perennial herb growing widely and used as traditional medicine in India, China and Bangladesh. The divine herb has potent medicinal values for the treatment of different type of illnesses. The HPTLC techniques were used to separate active components from ethanolic extract of tuber part of A. indica. This examination was intended to designed a HPTLC fingerprint profile of crude extract of the plant in ethanol. A HPTLC method for the isolation of various active constituents in A. indica ethanolic extract have been developed and solvent system for quercetin the mobile phase used was toluene: ethyl acetate: formic acid (5:2:1) and for analysis of β-sitosterol the mobile phase used was chloroform: ethyl acetate: formic acid (6:4:1) . In the present investigation, HPTLC fingerprint of extract of dried tuber part of A. indica have been performed and the results demonstrated that important information for standardization. The HPTLC system for routine quality control of present species can be used for ethanolic extract and serve in qualitative, quantitative and was appropriate for standardization of the plant.
Résumé
OBJECTIVE@#To assess the complete nutritional profile and identification of bioactive components present in the hydro-ethanolic extract of Alocasia indica tuber.@*METHODS@#The proximate composition and vitamins were assessed from fresh tissue while mineral content was detected from the ash using inductively coupled plasma atomic spectrophotometer. For gas chromatography analysis, the tubers were shade dried and extracted with ethanol using Soxhlet apparatus for 72 h. The extract was dried using rotary evaporator and analyzed for active components.@*RESULTS@#The tuber was rich in carbohydrate, but marginal in protein content. However, it showed moderate amount of dietary crude fibre, very low fat content and sufficient source of ascorbic acid and alpha-tocopherol. The tuber was also found to contain all the essential micro and macro mineral elements. It especially served as a good source of potassium and calcium while moderate source of iron, zinc and magnesium. Gas chromatography analysis also revealed the presence of several components of biological value in the ethanolic fraction of the extract. The extract was basically found to be a good source of poly-unsaturated fatty acids and some amount of polyphenols.@*CONCLUSIONS@#All the major compounds identified and characterized by spectroscopic method were of biological significance. Besides, the tuber also possesses high calorific value and source for low fat and moderate dietary fibre which is essential for maintaining proper health. Moreover, the mineral content of the tuber can be used as supplement for combating malnutrition especially among rural folk and the vitamin content can serve as good source of natural antioxidant. Thus identification of a good number of important compounds from Alocasia indica tubers can focus on its use for future therapeutic purpose apart from maintaining general health.
Résumé
Objective: To assess the complete nutritional profile and identification of bioactive components present in the hydro-ethanolic extract of Alocasia indica tuber. Methods: The proximate composition and vitamins were assessed from fresh tissue while mineral content was detected from the ash using inductively coupled plasma atomic spectrophotometer. For gas chromatography analysis, the tubers were shade dried and extracted with ethanol using Soxhlet apparatus for 72 h. The extract was dried using rotary evaporator and analyzed for active components. Results: The tuber was rich in carbohydrate, but marginal in protein content. However, it showed moderate amount of dietary crude fibre, very low fat content and sufficient source of ascorbic acid and alpha-tocopherol. The tuber was also found to contain all the essential micro and macro mineral elements. It especially served as a good source of potassium and calcium while moderate source of iron, zinc and magnesium. Gas chromatography analysis also revealed the presence of several components of biological value in the ethanolic fraction of the extract. The extract was basically found to be a good source of poly-unsaturated fatty acids and some amount of polyphenols. Conclusions: All the major compounds identified and characterized by spectroscopic method were of biological significance. Besides, the tuber also possesses high calorific value and source for low fat and moderate dietary fibre which is essential for maintaining proper health. Moreover, the mineral content of the tuber can be used as supplement for combating malnutrition especially among rural folk and the vitamin content can serve as good source of natural antioxidant. Thus identification of a good number of important compounds from Alocasia indica tubers can focus on its use for future therapeutic purpose apart from maintaining general health.