The expression of annexin I and thymosin beta4 in cervical cancer / 대한산부인과학회지

OBJECTIVE: The aim of this study was to compare the expression of annexin I and thymosin beta4 in invasive cervical cancer including normal cervix and CIN. METHODS: In Ewha Womans University Mokdong Hospital, normal cervical tissues were obtained from healthy women (n=10), from patients with cervical intraepithelial neoplasia (CIN, n=10) and from patients with cervical cancer (n=33). The expressions of annexin I and thymosin beta4 mRNA and protein were examined by quantitative competitive-PCR and by western blot analysis. The expressions of annexin I and thymosin beta4 protein were measured by western blot analysis with thymosin beta4 peptides non treated and treated SiHa cells. RESULTS: The expression of thymosin beta4 mRNA and protein in cervical cancer were higher than that in normal cervix (p0.05). But thymosin beta4 and annexin I protein expressions were increased according to the cancer stage. The expression of annexin I was slightly higher in thymosin beta4 treated SiHa cells than that in nontreated SiHa cells. CONCLUSIONS: Our results suggest that overexpression of thymosin beta4 and annexin I may play roles in progression of invasive cervical cancer. Thymosin beta4 upregulates expression of annexin I in invasive cervical cancer. Therefore, thymosin beta4 and annexin I may be biological markers in detecting the progression of invasive cervical cancer, and their interaction is important in invasive cervical cancer.

Screening of differential expression proteins from human oral lichen planus and normal oral mucosa by two-dimensional difference gel electrophoresis and mass spectrometry / 实用口腔医学杂志

Objective:To find biomarkers for oral lichen planus by comparing differential expressing proteins. Methods:10 cases of oral lichen planus and normal oral mucosa tissues were collected.Total protein was extracted; differential proteome profiles were established and analyzed by two-dimensional polyacrylamide gel electrophoresis(2D-PAGE) and matrix-assisted laser desorption/ionization time of flight mass spectrometry (MALDI-TOF-MS). Results:(1)The well-resolved,reproducible 2-DE patterns of oral lichen planus and normal oral mucosa were obtained. The results showed that average protein spots were 1 576?67 and 1 608?73 in oral lichen planus and normal oral mucosa respectively, (2) The 13 differential protein spots were identified by Imaging Master 2D image analysis software between oral lichen planus and normal oral mucosa. There were 7 protein spots in oral lichen planus were higher than those in normal oral mucosa, 6 protein spots in oral lichen planus were lower than those in normal oral mucosa. 10 differential expressing proteins were analyzed by mass spectrometry and bioinformation. 4 of them were well characterized including manganese superoxide dismutase (Mn-SOD), Annexin I, vimentin and unknown proteins. Conclusion:Differential expression proteins might be candidate biomarkers for diagnosis of oral lichen planus;and proteomic technique is valuable for screening the diagnostic biomarkers.

Role of annexin-I in cervical cancer cell proliferation / 대한산부인과학회잡지

OBJECTIVES: To investigate the role of annexin-I in human cervical cancer, we evaluated the expression of annexin-I and the relation with the proliferation of cancer cells. Methods: By immunohistochemical analysis and the western blotting of annexin-I , we investigated the extent and distribution of the expression of annexin-I in cervical cancer tissues. After treating the human cancer cell lines ( SiHa and HeLa cell lines ) with tamoxifen, estradiol, and retinoic acid for 5 days to make the cells proliferate and antiproliferate, we measured the proliferation simultaneously with 3-(4,5- dimethyl thiazol- 2-yl)-2,5-diphenyl-tetr -azolium bromide (MTT) colorimetric assay and the expression level of annexin-I with flowcytometry. RESULTS: In the immunohistochemical stains, a granular staining pattern involving the entire cytoplasm was more heavily observed in malignant lesions than in normals. In the western blotting, the antibodies against 35-kDa annexin-I appeared to react more strongly with the lysates of cancer tissues than normal and benign tissues. In SiHa and HeLa cell lines with tamoxifen and beta- estradiol treatment, increased expressions of annexin-I were noted with correlated increased proliferation of cells, and with the treatments of all trans retinoic acid, decreased expressions of annexin-I were noted with correlated decreased proliferation of cells. CONCLUSIONS: The results suggest that the expression of annexin-I might correlate with cervical cancer than normal and the proliferation of cancer cells.