Chinese materia medica-A natural bioactive oligopeptides bank / 中草药

Oligopeptides are simple in composition, high in safety index and good in drug preparation, which are important components of biomedicine. However, due to the limitation of oligopeptide drug design theory and discovery pathways which are far less than other structural drugs, obtaining new bioactive oligopeptides is a hot and difficult topic in this field. As a natural oligopeptide library, Chinese materia medica (CMM) contains a large number of active oligopeptides. Oligopeptides of CMM have vital biological activities such as neuroprotection activity, liver protection activity, antitumor, anticoagulation, anti-oxidation, immunity enhancement, etc. In this paper, the sequence and biological activity of active oligopeptides discovered by scholars at home and abroad in the past 10 years were reviewed, and the relationship between active oligopeptides and classification of CMM was explored. It is preliminarily suggested that CMM such as tonifying deficiency drugs and promoting blood circulation and removing blood stasis are the sources of natural oligopeptides. There are many research results easy to be successful in the research and development of new oligopeptide drugs, which provides directions and ideas for the development of new active oligopeptide drugs.

Extraction and purification of C-phycocyanin from dry Spirulina powder and evaluating its antioxidant, anticoagulation and prevention of DNA damage activity.

C-Phycocyanin (C-PC) is a phycobiliprotein found in blue green algae, such as Spirulina platensis, is often used as a dietary nutritional supplement and exhibit a variety of pharmacological properties. In this regard, extraction, partial purification and antioxidant, anticoagulation and prevention of DNA damage activity of C-PC was investigated. In the present study, a simple and efficient method to extract C-PC from Spirulina platensis dry powder is reported. The extractions were carried out using two different methods: cold maceration and sonication method. The extraction using cold maceration method proved to be the most efficient method. Obtained crud CPC was purified by ammonium sulphate precipitation, dialysis and gel filtration and presented a final extraction yield of 3.27±0.09 mg/ml with a purity ratio of 2.317±0.08. When it was evaluated as an antioxidant in vitro, it was able to scavenge nitric oxide. C-PC showed significant anticoagulation and prevention of DNA damage activity.