Study on the Mechanism of Artemisia capillaries Based on Systems Pharmacology / 中国药房

OBJECTIVE:To investigate the possible mechanism of Artemisia capillaries,and to provide reference for further development and utilization of it. METHODS:The effective components and related target protein of A. capillaries were screened by Traditional Chinese Medicine Systems Pharmacology (TCMSP) analysis platform database. The effective compound-target protein visual network of A. capillaries was established by using Cytoscape 3.5.1 software,topology analysis was also performed. The protein-protein interaction (PPI) network was constructed and analyzed by STRING database. KEGG pathway enrichment of target protein coding gene was analyzed by DAVID bioinformatics resource database. RESULTS:A total of 13 kinds of effective compounds,189 target proteins and 34 enrichment pathways were selected. Quercetin,β-glutamol,isorhamnetin and artepillin C were main effective compounds. Prostaglandin G/H sythase 2(PTGS 2),heat shock protein 90(HSP 90),dipeptidyl peptidase Ⅳ, protein kinase A catalytic subunit Cα were main target proteins. Transcription factor AP-1 and cell tumor antigen p53 played a key role in PPI network. The target protein coding gene was rich in TNF-α signaling pathway,HIF-1 signaling pathway,Toll-like receptor signaling pathway,PI3K/Akt signaling pathway,T cell receptor signaling pathway,thyroid hormone signaling pathway, apoptotic signaling pathway,etc. CONCLUSIONS:Quercetin,β-glutamol and isorhamnetin of A. capillaries play an effect on PTGS2,HSP90,transcription factor AP-1 and other target proteins through TNF-α signaling pathway,HIF-1 signaling pathway and PI3K/Akt signaling pathway,so as to play anti-inflammatory and antitumor effect.

Effect of Artemisia capillaries Thunb on fatty liver rats with insulin resistance / 中成药

AIM: To study the liver protective hypolipidemic function of Artemisia capillaries Thunb on the fatty liver rats with insulin resistance of action. METHODS: Adult SD rats were maintained by high-fat-sugar-salt diet for 8 weeks,and extraction of Artemisia capillaries Thunb(high dosage 6.9 g/kg,low dosage 2.3 g/kg) was administered to the rats for 4 weeks by intragastric administration at the begining of 9~(th) week.And then all animals were killed and the blood was sampled to measure the levels of blood glucose,blood lipid(TC,TG,HDL-C,LDL-C,FFA),fasting serum insulin and the levels of MDA and TGF-?_1.The activities of ALT,AST and SOD also were measured.The liver was weighed and collected to observe the pathological changes. RESULTS: Compared with model rats,the levels of blood glucose and insulin decreased significantly in the treatment of Artemisia capillaries Thunb,and insulin sensitivity index returned to normal condition(P