Résumé
Objective: To observe the relieving cough and reducing sputum effects of total alkaloid in Atalantia buxifolia and evaluate the safety preliminarily.Methods: The relieving cough and reducing sputum effects of total alkaloid in Atalantia buxifolia were studied by the cough model caused by the irritation of ammonia water and the phenol red output of trachea in mice.The acute toxicity test and maximum tolerance test were carried out to evaluate the safety.Results: The total alkaloid in Atalantia buxifolia at low dose could obviously prolong cough incubation period and decrease cough times in mice, and that at high dose could significantly increase the secretion of phenol red in respiratory tract, and compared with those in the blank group, the differences were statistically significant (P0.05).Conclusion: The relieving cough and reducing sputum effects of total alkaloid in Atalantia buxifolia are notable in the cough model caused by the irritation of ammonia water and the phenol red output of trachea in mice.The maximum tolerable dose test shows the total alkaloid in Atalantia buxifolia is safe.
Résumé
Objective: To evaluate the effect of the ethanol extract and active parts from the roots of Atalantia buxifolia on chronic bronchitis (CB) and their mechanism. Methods: The rat model of CB was established with smoking method of sulfur dioxide, ethanol extract and active parts from the roots of A. buxifolia were ig administrated, the pathological changes of lung tissue in rats were analyzed, and the level changes of interleukin-10 (IL-10), interleukin-4 (IL-4), and tumor necrosis factor-α (TNF-α) were determined by ELISA method. Results: The administration of ethanol extract and n-butanol extract could improve the tiredness and weakness of CB rats, reduced food intake, and body weight without increasing status, decrease the degree of infiltration, epithelial cell shedding, bronchial secretion, inhibit the proliferation of bronchial epithelial cells of the rat bronchial inflammatory cells, and make the bronchial diameter increases and ventilation. The low dose of the ethanol extract and n-butanol extract could significantly inhibit the increase of TNF-α, IL-10, and IL-4 in the lung tissue of rats with CB in the homogenate, compared with the model group with the statistical significance (P0.05). Conclusion: The alcoholic extract and n-butanol extract in the roots of A. buxifolia have the better treatment effect on CB, which may be related to the balance of cytokine production related.