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1.
Hig. aliment ; 27(226/227): 179-182, 30/12/2013. ilus, tab
Article Dans Portugais | LILACS | ID: biblio-964261

Résumé

Listeria monocytogenes é uma crescente preocupação para a indústria devido à sua capacidade de crescer em temperatura de refrigeração durante o armazenamento de alimentos. Falhas na erradicação dessa bactéria podem resultar em grandes perdas econômicas e sérios problemas de saúde pública. Este trabalho teve por objetivo analisar 32 cepas de L. monocytogenes isoladas de diferentes alimentos frente a dois sanitizantes alcalino clorados em diferentes concentrações e tempos de exposição. Os resultados mostraram que o produto A, na concentração recomendada pelo fabricante, eliminou 46,8% das cepas no tempo mediano de 30 minutos. Já o produto B, eliminou 50% das cepas no tempo mediano de 15 minutos. Concluímos que apesar de estar em suspensão a bactéria mostrou certa resistência ao produto alcalino clorado e o tempo de exposição representa um fator determinante para eficácia do produto.


Listeria monocytogenes is a growing concern for the industry due to its ability to grow at refrigeration temperature for food storage. Failures in the eradication of this bacterium can result in large economic losses and serious problems in public health. This study aimed to analyze 32 strains of L. monocytogenes isolated from different foods in front of two chlorinated alkaline sanitizers at different concentrations and exposure times. The results showed that the product A at the concentration recommended by the manufacturer, removed 46.8% of the strains in the median time of 30 '. Have the product B, eliminated 50% of the strains at the median of 15 '. We conclude that despite being in the suspended bacteria showed some resistance to the chlorinated alkaline product and exposure time is the determining factor for product effectiveness.


Sujets)
Désinfectants , Stockage des aliments , Listeria monocytogenes , Industrie alimentaire , Emballage de produit , Produits Avec Action Antimicrobienne , Analyse d'aliment , Microbiologie alimentaire
2.
Acta cient. Soc. Venez. Bioanalistas Esp ; 10(1): 33-38, 2007. tab, graf
Article Dans Espagnol | LILACS | ID: lil-733464

Résumé

Dentro de los mecanismos de resistencia bacteriana encontrados en los miembros de las Enterobacteriaceae, las Beta Lactamasas de Espectro Expandido (BLEE), juegan un papel importante ya que debido a su naturaleza plasmídica pueden diseminarse a otros géneros diferentes a E. coli, K. pneumoniae y K. oxytoca, de donde principalmente se han descrito y confieren resistencia a cefalosporinas de tercera y cuarta generación y a aztreonam. Dado que en la Maternidad “Concepción Palacios” (MCP) la mayor parte de los pacientes constituyen neonatos y el tratamiento empleado principalmente son cefalosporinas, se evaluó la frecuencia en este centro de Enterobacterias productoras de BLEE aislados en áreas críticas de muestras de hemocultivos, puntas de catéter, orina y secreciones en el período comprendido de Enero a Junio de 2006. La identificación y sensibilidad se realizaron con las galerías ID32GN y ATBGN-5 respectivamente, utilizando el equipo semiautomatizado Mini API (BioMérieux). Para la detección fenotípica de producción de BLEE se empleó el método de doble difusión con discos y el método recomendado por el Clínical and Laboratory Standard Institute (CLSI) (1). La mayor frecuencia se obtuvo para Pantoea agglomerans, seguida de Klebsiella pneumoniae, y Enterobacter cloacae. El 80 % de las enterobacterias aisladas, fueron productoras de BLEE.


Inside the mechanisms of bacterial resistance found in members of the Enterobacteriaceae family, the Expanded Spectrum Beta Lactamasas (ESBL), play an important role due to its plasmid nature it can be spread to other different genera to E. coli, K.pneumoniae, and K. oxytoca, wherefrom principally they have been described, and award resistance to cephalosporin of third and fourth generation and to aztreonam. In view of the Maternity “Concepción Palacios” (MCP) most of the patients constitutes newborn, and the principally used treatment are cephalosporin, the frequency of ESBL producting enterobacteria was evaluated in this center, isolated in critical areas from samples of blood cultures, tops of catheter, urine and secretions from January to June, 2006. The identification and sensibility were realized by the galleries ID32GN and ATBGN-5 respectively, using the semi automated Mini API (BioMérieux) equipment. For the phenotypical detection of ESBL production there were used the double diffusion of disc method and the method recommended by the Clinical and Laboratory Standard Institute (CLSI) (1). The major frequency was to Pantoea agglomerans followed by Klebsiella pneumoniae, and Enterobacter cloacae. 80% of the isolated enterobacteria, were producer of ESBL.


Sujets)
Humains , Mâle , Femelle , Nouveau-né , Antibactériens/usage thérapeutique , Céphalosporines/usage thérapeutique , Résistance microbienne aux médicaments , Enterobacteriaceae/pathogénicité , Pantoea/pathogénicité , bêta-Lactamases/usage thérapeutique , Bactériologie
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