Protective effect of Xiebaisan on allergic asthma in rats based on non-targeted metabolomics and intestinal bacterial flora / 中国实验动物学报

Objective To explore the possible mechanism of Xiebaisan in protecting against allergic asthma in rats from the perspective of host intestinal flora metabolism.Methods SPF SD rats were divided into normal group(NC group),model group(M group),and Xiebaisan group.The allergic asthma rat model was established by ovalbumin.Changes in lung histopathology were observed by HE staining.Colon contents were harvested for 16S rDNA high-throughput sequencing to assess changes in the intestinal flora structure and function.Serum and lung tissue samples were collected for non-targeted metabolomics by Ultra-high performance liquid-time-of-flight mass spectrometer.Results HE staining showed some improvement of lung histomorphology in asthmatic rats in the Xiebaisan group compared with that in the M group.16S rDNA high-throughput sequencing showed that the diversity of intestinal flora was decreased in the M group and increased in the Xiebaisan group compared with the M group,the microecosystem of intestinal was improved.Non-targeted metabolomics of serum showed regulation of amino acid metabolism and the mTOR pathway in the Xiebaisan group,and partially reversed differential metabolite expression in the M group.Non-targeted metabonomics of lung tissue samples showed regulation of carbon metabolism,vascular smooth muscle and cAMP signaling pathways in the Xiebaisan group,and partially reversed differential metabolite expression in the M group.Conclusions The protective effects of Xiebaisan on allergic asthma in rats may be related to improvement of the morphological structure of lung tissue,the diversity of intestinal flora,and regulation of mTOR,vascular smooth muscle contraction,and cAMP pathways,which affect amino acid and carbon metabolism.

Characteristics of faecal bacterial flora and volatile fatty acids in Min pig, Landrace pig, and Yorkshire pig

BACKGROUND In order to study the influence of long-term growth process and evolution environment on intestinal bacteria of different breeds, the intestinal bacteria and volatile fatty acids among the faeces of Min, Landrace and Yorkshire pigs were analysed by Illumina high-throughput sequencing of the 16S-rDNA and gas chromatography. RESULTS The shared core microbiota of Landrace, Yorkshire and Min pig were 1273, accounting for 69.56% of total abundance of organisms. The proportion of Firmicutes in Min pig faeces (57.89%) was significantly higher than that in Landrace and Yorkshire pig faeces (47.01% and 46.40%, respectively) (P < 0.05), but that of Bacteroidetes was exactly opposite. Moreover, Min pig presented more highly efficient membrane transport, environmental adaptation, carbohydrate transport, and metabolism than Yorkshire pig (P < 0.05). The acetic acid/total volatile fatty acid ratio in Min pig was significantly higher than that in Landrace pig (P < 0.05), and the isobutyric acid/ total volatile fatty acid ratio in Min pig was significantly larger than that in Yorkshire pig (P < 0.05). Furthermore, the content of branched chain volatile fatty acids in Min pig was significantly higher than that in Yorkshire pig (P < 0.05). CONCLUSION This study demonstrated that Min pig, as an excellent breed in the cold area of China, possessed special intestinal floral structure compared to the imported pigs in order to satisfy their phys iological and metabolic demands, which may influence their characteristics such as resistance to cold, diseases, and crude feeding, and the ability to deposit intramuscular fat.

Correlation between changes of oral bacterial flora and radiation-induced oral mucositis during radiotherapy for head and neck / 中华放射肿瘤学杂志

Objective:To investigate the correlation between the changes of oral bacterial flora during head and neck radiotherapy and radiation-induced oral mucositis (ROM).Methods:The oral bacterial samples of patients with nasopharyngeal carcinoma and accompanying family members were obtained before and at the end of radiotherapy and subjected to high-throughput sequencing. C57BL/6 mice were used to establish the ROM models. On the 9 th day after radiotherapy, oral bacterial samples were collected in the radiotherapy group and the negative control group. On the 3 rd, 5 th, 7 th, and 9 th days post-radiotherapy, the tongue tissues were obtained from another batch of mice in the negative control and radiotherapy groups. Inflammatory factors were detected with PCR and HE staining was performed. Results:The oral bacterial diversity of patients after radiotherapy significantly differed from that of patients before radiotherapy and their accompanying family members before and after radiotherapy in Observed species, Chao1, Simpson index (all P<0.05). There was a significant difference in Shannon index between the severe and mild ROM patients ( P=0.036). LEfSe analysis showed that patients with severe ROM had higher levels of g_ Streptococcus and f_ Streptococcus, and lower levels of f_ Familyxl, g_ Gemini and o_ Bacillus. The Simpson index and PCoA results in the oral bacterial samples significantly differed between the negative control and radiotherapy groups (all P<0.05). Conclusions:Radiotherapy can disrupt the balance of bacterial flora. The dysregulated oral bacterial flora is closely associated with the aggravation of ROM.

Characteristics of skin microbiota in an atopic dermatitis-like mouse model induced by 2,4-dinitrochlorobenzene / 中华皮肤科杂志

Objective:To investigate characteristics and changes of skin microbiota in atopic dermatitis-like mouse models induced by different concentrations of 2,4-dinitrochlorobenzene (DNCB) .Methods:Totally, 30 male specific-pathogen-free BALB/c mice were randomly divided into 3 groups by using a random number table: negative control group topically treated with 200 μl of mixture of acetone and olive oil at a volume ratio of 3∶1 on the back twice a week for 6 consecutive weeks; high-and low-concentration DNCB groups both topically treated with 200 μl of 1% DNCB on the first and third day at the first week, followed by topical application of 200 μl of 0.5% and 0.1% DNCB, respectively, twice a week for 5 weeks from the second week. Twenty-four hours after the last treatment, the severity of skin lesions was evaluated, and the transepidermal water loss and stratum corneum hydration were measured. After the experiment, the mice were sacrificed, and skin tissues were resected from the back of the mice for histopathological examination. Full-thickness skin tissue samples were obtained from the back of 3 mice in each group. Illumina Miseq PE300 high-throughput sequencing was performed to sequence the V3-V4 variable region of 16S rRNA gene of skin microbiota on the back of the mice, and the composition and structure of the skin microbiota and changes in the relative abundance of different genera were analyzed. One-way analysis of variance was used to analyze differences in indices among the 3 groups, and the Games-Howell method was used for multiple comparisons.Results:The severity scores of skin lesions were significantly higher in the high-and low-concentration DNCB groups (9.83 ± 2.45 points, 2.71 ± 0.56 points, respectively) than in the negative control group (0.51 ± 0.12 points, t=-7.19,-2.85, respectively, both P < 0.05) . Compared with the negative control group, the high-and low-concentration DNCB groups showed significantly increased transepidermal water loss ( t=-7.72,-2.68, respectively, both P < 0.05) , but significantly decreased stratum corneum hydration ( t=6.77, 5.99, respectively, both P < 0.05) ; the transepidermal water loss was significantly higher in the high-concentration DNCB group than in the low-concentration DNCB group ( t=2.76, P < 0.05) , while no significant difference in the stratum corneum hydration was observed between the high-and low-concentration DNCB groups ( P > 0.05) . There was a significant difference in the relative abundance of Corynebacterium among the 3 groups ( F=249.85, P < 0.001) , which was highest in the high-concentration DNCB group. No significant differences in the observed species and Chao1 index of the skin samples were observed among the 3 groups (both P > 0.05) , and the Shannon index was significantly lower in the high-concentration DNCB group than in the low-concentration DNCB group and negative control group ( t=6.96,-6.37, respectively, both P < 0.05) . Conclusion:DNCB could induce atopic dermatitis-like dermatitis in mice, and the severity of skin lesions and degree of barrier function impairment were related to the concentration of DNCB; the species diversity of skin microbiota markedly decreased in the high-concentration DNCB group, indicating that high-concentration DNCB modeling has more advantages in studying microbiological changes associated with atopic dermatitis.

The human gut resistome: Current concepts & future prospects

The human gut is home to a myriad of organisms. While some are harmless commensals, others are transient, pathogenic flora. The gut microbiome is composed of diverse bacterial flora, and apart from playing a major role in protecting from various infectious and non-infectious diseases, it plays an important role in resistance to antimicrobials. The collection of genes or genetic material that confers antimicrobial resistance constitutes the gut resistome, and it may involve the pathogens or commensals of the intestinal tract. The diversity of this gut resistome is influenced by various environmental factors including the diet and antibiotic exposure. This review highlights the recent concepts pertaining to the human gut resistome, factors affecting it, how it impacts human health and diseases, methods to study the resistome and potential therapeutic approaches.

Current Trends of Surface Versus Core Bacterial Flora among Recurrent Tonsillitis Patients in a North Indian Tertiary Care Hospital

Introduction: Recurrent tonsillitis is a major cause of morbidity and may require surgical intervention when medical treatment has failed. Reasons suggested for treatment failure include the use of inappropriate antibiotics aimed at pathogens present on the tonsil surface rather than those present in the tonsil core. Material and Methods: A total of 60 cases of chronic and recurrent tonsillitis which were not responding to medical treatment and are fit and willing for surgery were included in the study. Results: The culture results of tonsillar surface and core tissue revealed that the majority of samples showed pathogens. Out of 120 cultures of tonsillar tissue,14 results showed the growth of commensal flora. Single pathogen was isolated from 86 samples from surface and core of the tonsil. However, 14 cultures of core tissue revealed growth of two pathogens as compared to 6 cultures from the tonsillar surface. Conclusion: Staphylococcus is the most common pathogen isolated from the tosillar surface as well as core followed by GABHS

Vaginal Bacterial Profile in Buffaloes Following Treatment with Progesterone Insert

The objective of this study was to characterize the vaginal bacterial flora and subsequent conception rates after AI in buffaloes subjected to 3 different estrous induction regimes-the use of CIDR and use of two intravaginal sponges (Polyurethane sponges with micronized progesterone and Polyurethane sponges with micronized progesterone with Carboxy methyl cellulose). The estrus induction was 100% in Group I and II followed by 91.67 % in group III. The pregnancy rates were Group I, II and III were 50.00, 66.67 and 54.55% respectively. All the vaginal swabs in all three groups yielded growth of bacteria and the predominance of mixed isolates over single isolates 81.94% vs 18.06% was indicative of dominance of mixed culture over single isolates. E. coli, Staphylococcus, Proteus and Klebsiela spp. were the commonest isolates obtained prior to insertion and after removal of implants in postpartum anestrus buffaloes. The gram positive bacteria were Staphylococcus, Streptococcus and Bacillus spp. while, gram negative bacteria were E. coli, Proteus and Klebsiella spp. These organisms could be considered as a part of the normal bacterial flora of the buffalo

Effect of long-term application of prostaglandin analogue eye drops on conjunctival flora in glaucoma patients / 国际眼科杂志(Guoji Yanke Zazhi)

@#AIM: To investigate the effect of long-term application of prostaglandin analogue eye drops on conjunctival flora in glaucoma patients.<p>METHODS: Totally 69 patients(113 eyes)with glaucoma treated with latanoprostaglandin from June 2016 to June 2017 were selected, and the sensitivity of conjunctival flora and staphylococcus epidermidis to antibiotics was examined after admission, 3mo and 1a.<p>RESULTS: The number of bacterial eyes detected at admission, 3mo and 1a were 63, 66 and 67 respectively(χ²=2.496, <i>P</i>=0.287). There was no difference in the detection rates of staphylococcus epidermidis, streptococcus, staphylococcus aureus, staphylococcus hominis, staphylococcus hemolyticus and moraxella at the time of admission, 3mo and 1a(<i>P</i>>0.05). The ratio of MRSE at 3mo was higher than that at entry(χ²=3.063, <i>P</i>=0.062). The ratio of MRSE at 1a was higher than that at 3mo(χ²=38.945, 24.984, all <i>P</i><0.001). The susceptibility of staphylococcus epidermidis to levofloxacin, gatifloxacin, moxifloxacin, ceftazidime, tobramycin, cefmethime and erythromycin at 3mo was lower than that at the time of admission, but there was no difference(<i>P</i>>0.0167), while the susceptibility of staphylococcus epidermidis to chloramphenicol at 3mo was lower than that at entry(<i>P</i><0.0167). The sensitivity of staphylococcus epidermidis to levofloxacin, gatifloxacin, moxifloxacin, ceftazidime, cefmethime, tobramycin, chloramphenicol and erythromycin at 1a was lower than that at 3mo(<i>P</i><0.0167).<p>CONCLUSION: The long-term application of latanoprostaglandin will not affect the detection rate of conjunctival flora, but can increase the incidence of MRSE. Clinically, patients who need long-term application of latanoprostaglandin should be considered comprehensively to prevent the emergence of drug-resistant strains in order to prevent uncontrollable infection.

Microbiota intestinal y la dieta en el tratamiento de la enfermedad inflamatoria intestinal / Intestinal microbiota and diet in the treatment of inflammatory bowel disease

RESUMEN Fundamento: La enfermedad inflamatoria intestinal comprende la colitis ulcerativa idiopática y la enfermedad de Crohn. En la patogenia intervienen factores genéticos y ambientales como la alteración de las bacterias luminales y el aumento de la permeabilidad intestinal, factores que alteran la inmunidad intestinal, causas estas de lesión gastrointestinal. Objetivo: Analizar la influencia de la dieta en la microbiota intestinal en la enfermedad inflamatoria intestinal, así como concientizar a los profesionales de la salud en la importancia de la terapia a partir de la dieta, como pilar esencial en el control de esta enfermedad digestiva crónica. Metodología: Se realizó una búsqueda en bases de datos como: Scielo, Pubmed/Medline, Ebsco, Clinical Key, Springer, Web of Science, Infomed, se incluyeron revistas, libros, repositorios de tesis, sitios web de especialidades. Desarrollo: En los pacientes con enfermedad inflamatoria intestinal existe una disbiosis que contribuye potencialmente a una respuesta inmune proinflamatoria. Conclusiones: El equilibrio entre el huésped y su microbiota intestinal es esencial para el desarrollo inmunológico óptimo; la modificación de la dieta y la flora bacteriana intestinal son dianas potenciales en el tratamiento y prevención de la misma.

ABSTRACT Background: Inflammatory bowel disease includes idiopathic ulcerative colitis and Crohn's disease. In the pathogenesis are present genetic and environmental factors such as alteration of luminal bacteria and increased intestinal permeability, factors that alter the intestinal immunity, these causes of gastrointestinal injury. Objective: To analyze the influence of diet on intestinal microbiota in inflammatory bowel disease, as well as to increase awareness among health professionals about the importance of diet-based therapy as an essential pillar in the control of this chronic digestive disease. Methodology: A search was made in databases such as: Scielo, Pubmed / Medline, Ebsco, Clinical Key, Springer, Web of Science, Infomed, magazines, books, thesis repositories, and specialty websites. Development: In patients with inflammatory bowel disease there is a dysbiosis that potentially contributes to a proinflammatory immune response. Conclusions: The balance between the host and its intestinal microbiota is essential for the optimal immunological development; the modification of the diet and the intestinal bacterial flora are potential targets in the treatment and prevention of it.

Changes in the conjunctival bacterial flora of patients hospitalized in an intensive care unit / Mudanças na flora bacteriana conjuntival de pacientes internados em unidade de terapia intensiva

ABSTRACT Purpose: To identify the changes in aerobic conjunctival bacterial flora and to correlate culture results with physical health and the duration of patients' hospitalization in an intensive care unit (ICU). Methods: Patients hospitalized in the ICU were included in this study. Conjunctival cultures from all patients were obtained using a standard technique on days 1, 3, 7, and 14. Swabs were plated on nonselective (blood agar) and enriched (chocolate agar) media within one hour. Visible colonies were isolated, and standard microbiological techniques were used to identify the bacteria. The frequency, identity, and correlation of culture results with patients' physical findings and the duration of hospitalization were determined. Results: We obtained 478 cultures (day 1, 270; day 3, 156; day 7, 36; and day 14, 16) from 135 patients; 288 (60.2%) cultures were positive, and 331 microorganisms were isolated. The most frequently isolated microorganism from the cultures was coagulase-negative Staphylococcus species (n=210/331, 63.5%), and the others were Corynebacterium diphtheriae (n=52/331, 15.7%), S. aureus (n=26/331, 7.9%), gram-negative bacilli other than Pseudomonas (n=14/331, 4.2%), Neisseria species (n=8/331, 2.4%), Pseudomonas aeruginosa (n=6/331, 1.8%), Haemophilus influenzae (n=7/331, 2.1%), Acinetobacter species (n=6/331, 1.8%), and Streptococcus species (n=2/331, 0.6%). The frequency of positive cultures significantly increased (p<0.03) with time. Conclusions: Prolonged hospitalization significantly predisposes to bacterial colonization. The colonization rate of S. aureus and Neisseria spp. increased significantly after one week.

RESUMO Objetivo: Identificar as mudanças na flora bacteriana aeróbia da conjuntiva e correlacionar os resultados da cultura com o estado de saúde física e a duração da hospitalização em pacientes em uma unidade de terapia intensiva (UTI). Método: Pacientes que estavam na UTI foram incluídos neste estudo. Culturas conjuntivais foram obtidas nos dias 1, 3, 7 e 14 de todos os pacientes com uma técnica normalizada. Zaragatoas foram semeadas em placas não seletivas (ágar sangue) e enriquecidas (ágar chocolate) dentro de uma hora. Colônias visíveis foram separadas, isoladas, e identificadas utilizando técnicas microbiológicas convencionais. A frequência, identificação e correlação da cultura resulta com achados físicos e a duração da hospitalização foram determinados. Resultados: Um total de 478 culturas (no primeiro dia 270, terceiro dia 156, sétimo dia 36 e dia catorze 16 culturas) foram obtidas de 135 pacientes hospitalizados durante o estudo. Duzentos e oitenta e oito (60,2% de todas as culturas obtidas) culturas foram positivas. Trezentos e trinta e um microrganismos foram isolados a partir dessas culturas. Em todos os grupos, o microrganismo mais frequentemente isolado foi o Staphylococcus species coagulase negativo (n=210/331, 63,5% de todos os microrganismos isolados). Outras bactérias isoladas foram Corynebacterium diphteriae (n=52/331, 15,7%), Staphylococcus aureus (n=26/331, 7,9%), bacilos Gram-negativos que não sejam Pseudomonas (n=14/331, 4,2%), Neisseria species (n=8/331, 2,4%), Pseudomonas aeruginosa (n=6/331, 1,8%), Haemophilus influenzae (n=7/331, 2,1%), Acinetobacter species (n=6/331, 1,8%), e Streptococcus species (n=2/331, 0,6%). Como o tempo de hospitalização prolongada, a positividade em culturas aumentou significativamente (p<0,03). Conclusões: hospitalização prolongada predispõe significativamente a frequência de colonização bacteriana. A taxa de colonização de S. aureus e Neisseria spp. aumentou significativamente depois de uma semana.

Analysis in saliva microbiome composition in patients with NAFLD and normal subjects / 实用医学杂志

Objective To analyze the diversity of salivary bacteria in patients with nonalcoholic fatty liv-er disease(NAFLD group)and healthy control group(HC group). Methods Saliva samples were collected from 24 patients with nonalcoholic fatty liver disease and 22 healthy control.Genomic DNA of the samples was extracted and extended to 16S rRNA V4-V5 hypervariable region after PCR with Illumina high-throughput sequencing tech-nology.All the data,including operational taxonomic units,diversity indexes and species annotation,etc.was ana-lyzed. Results 39118 optimized sequences in the saliva of each group were obtained,including nonalcoholic fatty liver group clustering OTUs about 308 ± 48 and healthy control group clustering OTUs about 305 ± 53.The diversi-ty analysis of NAFLD group and HC group showed that there was little difference in diversity of the whole salivary bacteria.The data of the species analysis of the saliva bacteria in two group indicate that the composition was simi-lar and the relative abundance was different in phylum and genus.In NAFLD group,the ratio of the abundance of firmicutes(20.42%)increased and the proportion of bacteroides(36.75%)decreased in phylum. Genus of the NAFLD group,the ratio of fusobacterium,porphyromonas and veillonella increased significantly,and the ratio of prevotella and[Prevotella]decreased.Conclusion In the NAFLD group,the abundance of firmicutes increased, and the abundance of the bacteria decreased.It was speculated that the imbalance of the salivary bacteria is associ-ated with nonalcoholic fatty liver disease.

Bacterial culture and antibiotic sensitivity from the ocular conjunctiva of horses / Cultura e sensibilidade bacteriana da conjuntiva ocular de equinos

ABSTRACT: The aim of this study was to identify the conjunctival bacterial flora of healthy horses in Brasilia (Distrito Federal, Brazil), and to evaluate the antimicrobial susceptibility of the isolated strains. We examined 200 eyes of 100 healthy adult horses without any eye problems, belonging to the 1st Regiment of the Cavalry Guard (RCG) of the Brazilian Army in Brasilia. Samples were collected from the inferior conjunctival fornix of both eyes. Drug sensitivity test was performed with the antibiotics gentamicin (10µg), tobramycin (10µg), chloramphenicol (30µg) and ciprofloxacin (5µg). Of the 200 samples collected, 131 (65.5%) were considered positive for bacterial growth. A total of 208 bacterial strains belonging to 19 genera were isolated, where there was prevalence of gram-positive bacteria (65%), with Staphylococcus sp. being the species of greatest incidence. It was observed that 94, 85, 81 and 68% of the isolates were sensitive to ciprofloxacin, gentamicin, chloramphenicol and tobramycin, respectively. These results can guide the empirical selection antimicrobial therapy for infections of the ocular surface of horses, pending the identification of the etiologic agent.

RESUMO: Objetivou-se identificar a flora bacteriana conjuntival de cavalos saudáveis, residentes de Brasília (Distrito Federal - Brasil), e avaliar a susceptibilidade antimicrobiana das cepas isoladas. Foram avaliados 200 olhos de 100 equinos hígidos, adultos, saudáveis e sem alterações oculares, provenientes do 1o Regimento de Cavalaria de Guarda (RCG) do Exército de Brasília. As amostras foram coletadas do fórnix conjuntival inferior de ambos os olhos. O teste de sensibilidade foi realizado para os antimicrobianos: gentamicina (10mcg), tobramicina (10mcg), cloranfenicol (30mcg) e ciprofoxacino (5mcg). Das 200 amostras coletadas, 131 (65,5%) foram consideradas positivas para o crescimento bacteriano. Um total de 208 bactérias pertencentes a 19 gêneros foram isoladas, verificando-se prevalência de bactérias gram-positivas (65%), sendo Staphylococcus sp, a espécie de maior incidência. Foi observado que 94%, 85%, 81% e 68% das bactérias isoladas foram sensíveis ao ciprofloxacino, à gentamicina, ao cloranfenicol e à tobramicina, respectivamente. Tais resultados podem direcionar a escolha empírica da terapêutica antimicrobiana nas afecções da superfície ocular de equinos, enquanto se aguarda a identificação do agente etiológico.

Role and Therapeutic Prospect of Intestinal Microecology in Crohn’s Disease / 胃肠病学

The etiology and pathogenesis of Crohn’s disease(CD)are not fully clear,and genetic susceptibility, immunologic disorder,intestinal barrier dysfunction and intestinal microecology are considered to be involved in the pathogenic mechanism of CD. In recent years,the relationship between intestinal microecology and CD has received much attention. Several studies confirmed that the intestinal microecology in CD patients was different from that in normal person. The change of intestinal microecology was correlated with the occurrence of CD,and modulation of intestinal flora was effective in the treatment of CD. This article reviewed the relationship between intestinal microecology and CD and the therapeutic prospect of intestinal microecology for the treatment of CD.

The prevention and control measures of oropharyngeal flora distribution disorder in patients with chronic obstructive pulmonary disease / 国际检验医学杂志

Objective To observe the mild chronic obstructive pulmonary disease (COPD) patients′oropharyngeal flora distri‐bution state ,discuss the illness development and prevention measure of COPD patients with oropharyngeal flora disorder .Methods 124 cases of mild COPD patients from the medical center were enrolled in the study to do bacterial culture of swabs ,and 100 cases of healthy subjects were recruited at the same time to do bacterial culture of swabs as control group .The oropharyngeal bacteria distribution state was observed .Results The number of species and detection rates of main pathogenic bacteria and conditional pathogenic bacteria in COPD patients was higher than healthy subjects .In mild COPD patients ,the patients with abnormal oropha‐ryngeal bacteria isolates≥3 ,accounted for 29 .0% (36/124) which was significantly higher than that in control group(8 .0% ,8/100) and the difference was statistically significant(P< 0 .05) .Conclusion Because of immune factors and bad living habits such as smoking ,which could lead to the disorder of body′s normal flora ,infection and make the illness developed .Monitoring of oropharyn‐geal flora distribution state ,and taking active measures to prevent and control it had clinical significance in the prevention of infec‐tion and the development of mild COPD .

Analysis of bacterial flora in faeces and mucosa of sigmoid colon in patients with ulcerative colitis / 中华消化杂志

Objective To explore the difference in bacterial flora between faeces and mucosa of sigmoid colon,the possible role and significance of microbiota alteration in the genesis of ulcerative colitis (UC).Methods Fusobacterium, Enterococcus, Lactobacillus, Bifidobacterium, Bacteroides and Escherichia were selected as target bacteria colonies.The content of six target bacteria colonies in faeces and mucosa of sigmoid colon of 35 UC patients (20 active UC, and 15 UC in remission) and 20 health controls were detected by real-time fluorescent quantitative polymerase chain reaction (RT-PCR).Two independent samples t-test was performed to compare the differences in bacterial flora between faeces and mucosa of sigmoid colon.Variance analysis was used to compare the differences in bacterial flora among health controls group,active stage group and remission stage group.Results In health control group, the contents of Fusobacterium, Bacteroides, Enterococcus and Lactobacillus in faeces ((10.94 ± 0.29),(12.42±0.39), (8.73±0.84), (9.05±0.35), respectively) were higher than those in the mucosa of sigmoid colon ((9.36±0.66), (9.88±0.82), (7.70±1.17) and (7.96±0.68), respectively, t=9.83, 12.51, 3.20 and 6.35, all P＜0.05).However, the content of Escherichia was lower in faeces than that in the mucosa of sigmoid colon ((8.03±1.02) lg copy/g vs (8.91±0.52) lg copy/g, t=-3.44, P＜0.05).There was no difference in the content of Bifidobacterium between faeces and mucosa of sigmoid colon ((9.54±0.79) lg copy/g vs (9.42±0.98) lg copy/g, P＞0.05).For UC patients, the contents of Fusobacterium, Bacteroides, Enterococcus, Lactobacillus and Bifidobacterium in faeces ((9.62 ±± 1.13),(11.31±0.71), (9.33±0.65), (8.42±0.80) and (8.85±0.73) lg copy/g, respectively) were higher than those in the mucosa of sigmoid colon ((9.00±0.79), (8.80±0.66), (7.46±0.82), (6.82±1.07) and (8.40±0.72) lg copy/g, respectively, t=2.66, 15.28, 10.58, 7.12 and 2.56, all P＜0.05).The content of Escherichia was lower in faeces than that in the mucosa of sigmoid colon ((8.50 ± 0.52) lg copy/g vs (9.26±0.87) lg copy/g, t=-4.45, P＜0.05).Compared with health control group, the content of Fusobacterium, Lactobacillus, Bifidobacterium and Bacteroides ((8.83 ± 0.81), (7.48 ± 1.59), (8.55±0.79) and (11.11±0.88) lg copy/g) all decreased (F=84.45, 14.58, 10.43 and 24.91, all P＜0.05), while the contents of Enterococcus and Escherichia increased ((9.63 ± 0.39) and (8.74 ±0.53) lg copy/g, F=9.87 and 5.55,both P＜0.05).For remission stage group, only the content of Bacteroides decreased ((11.56±0.21) lg copy/g, P＜0.05).Compared with health control group, the contents of Fusobacterium, Bacteroides, Lactobacillus, Bifidobacterium ((8.52 ± 0.30), (8.34 ±0.29), (6.29±0.52) and (8.06±0.21) lg copy/g) all decreased in active stage group (F=16.99,35.98,22.28 and 16.08, all P＜0.05);the content of Escherichia increased ((9.68±0.56) lg copy/g, F=11.19,P＜0.05);there was no difference in the content of Enterococcus ((7.19±0.32) lg copy/g, P＞0.05).In remission stage group, the contents of Bacteroides fragilis and Bifidobacterium decreased ((9.42±0.48) lg copy/g and (8.87±0.89) lg copy/g, both P＜0.05), there was no difference in other bacterias (all P＞0.05).In both faeces and mucosa of sigmoid colon, the ratios of Bifidobacterium and Enterobacteriaceae (B/E value) in active stage group were less than 1 (0.98±0.13 and 0.84±0.05),which significantly decreased compared with health control group (1.21 ± 0.19, 1.06 ± 0.08;F=12.64,76.20, both P＜0.05).In remission stage group, B/E values were higher than 1 both in faeces and mucosa (1.14±0.08 and 1.02±0.04), and there was no difference compared with those of control group (P＞0.05).Conclusions The distribution of target bacteria in feces and sigmoid colonis differed between health controls and UC patients.There are obvious changes in fecal and mucosa associated bacterial flora in UC patients especially in active stage compared with healthy controls.

Characteristic of bacterial flora from the uterus in HanWoo cattle / 대한수의학회지

Uterine sterilization is important for improving fertility in cattle. This study compared bacterial flora in the uterus between healthy and repeat breeder cows (RBCs). The uterine flushing of six heifers, 13 healthy HanWoo cows and eight RBCs (HanWoo) were sampled, and 15 frozen semen samples were selected. Overall, 35 bacteria were identified from in HanWoo uterine flushing and semen. The bacterial genera identified from HanWoo uterine flushing were Alloiococcus, Bacillus, Enterobacter, Enterococcus, Erysipelothrix, Gardnerella, Granulicatella, Kocuria, Pantoea, Pasteurella, Rothia, Serratia, Sphingomonas, Staphylococcus, Stenotrophomonas and Streptococcus. The bacterial genera identified from HanWoo semen were Bacillus, Escherichia, Kocuria, Oligella, Pseudomonas, Serratia, Sphingomonas, Staphylococcus, Stenotrophomonas and Streptococcus. The prevalence and presence of the identified bacteria between healthy cows and RBCs differed significantly. Further studies are needed to determine the role of these bacteria in the uterus of HanWoo cattle with reproductive disorder.

Antimicrobial effects of natural extracts on bacteria isolated from the uterus of Hanwoo (Korean native cattle) / 대한수의학회지

Bacterial infection of the uterus in Hanwoo can kill embryos. Therefore, many antibiotics have been used to treat this infection in the uterus of repeat breeders. Incorrect use of antibiotics has led to resistance in bacteria. Natural compounds have used as substitutes for antibiotics because they are safe and have very mild side effects. This study was conducted to examine the antimicrobial effects of five extracts from medicinal plants including Humulus japonicas (Hj), Phelledendron amurense (Pa), Viola mandshurica (Vm), Carthamus tinctorius (Ct), and Chelidoni herba (Ch) on bacteria isolated from the uterus of Hanwoo using the paper disc diffusion method. Hj and Pa extracts had potent antimicrobial effects against Staphylococcus lentus, Streptococcus infantarius subsp. coli, and Bacillus pumilus. Pa had the greatest antimicrobial effect among the five medicinal plants and was effective against 19 types of bacteria from bovine uterus. Compared to Pa, Hj showed weaker antimicrobial effects on all the bacteria tested except Pseudomonas aeruginosa. Vm, Ct, and Ch also showed weak antimicrobial effects on the tested bacteria. The results obtained suggest that Hj and Pa are natural compounds suitable for treating bacterial infection in repeat breeders and improving conception rates of Hanwoo.

Antimicrobial effects of natural extracts on bacteria isolated from the uterus of Hanwoo (Korean native cattle) / 대한수의학회지

Bacterial infection of the uterus in Hanwoo can kill embryos. Therefore, many antibiotics have been used to treat this infection in the uterus of repeat breeders. Incorrect use of antibiotics has led to resistance in bacteria. Natural compounds have used as substitutes for antibiotics because they are safe and have very mild side effects. This study was conducted to examine the antimicrobial effects of five extracts from medicinal plants including Humulus japonicas (Hj), Phelledendron amurense (Pa), Viola mandshurica (Vm), Carthamus tinctorius (Ct), and Chelidoni herba (Ch) on bacteria isolated from the uterus of Hanwoo using the paper disc diffusion method. Hj and Pa extracts had potent antimicrobial effects against Staphylococcus lentus, Streptococcus infantarius subsp. coli, and Bacillus pumilus. Pa had the greatest antimicrobial effect among the five medicinal plants and was effective against 19 types of bacteria from bovine uterus. Compared to Pa, Hj showed weaker antimicrobial effects on all the bacteria tested except Pseudomonas aeruginosa. Vm, Ct, and Ch also showed weak antimicrobial effects on the tested bacteria. The results obtained suggest that Hj and Pa are natural compounds suitable for treating bacterial infection in repeat breeders and improving conception rates of Hanwoo.

Changes of mucosa associated bacterial flora in patients with ulcerative colitis at different stages and its significance / 安徽医科大学学报

Objective Sigmoid mucosa specimens of the patients with ulcerative colitis ( UC ) at active stage and remission stage were respectively detected by real-time PCR for the contents of the six kinds of bacterial floras inclu-ding fusobacterium, enterococcus, lactobacillus, bifidobacterium, bacteroides, and escherichia coli. So the possi-ble roles and significance of the changes of intestinal mucosa associated bacterial flora in the pathogenesis of UC were discussed. Methods Sigmoid biopsy tissues were collected from 35 UC patients ( 20 cases were activities group while 15 cases were remission group) and 20 healthy cases( control group) . Specific primers were set accord-ing to the bacterial 16 SrDNA sequences. Bacterial DNA of the intestinal mucosa specimens was extracted, and re-al-time PCR was used to detect the numbers of different bacterial colonies. Results In sigmoid mucosa specimens of the UC group at activities group, escherichia coli colony was increased, while bifidobacterium, bacteroides, lac-tobacillus and fusobacterium, were reduced compared to the control group(P0.05). And in remission group, bacteroides and bifidobacterium were reduced com-pared with the control group(P0.05 ) . The ration of bifidobacterium to escherichia coli ( B/E ) in UC pa-tients at active stage was less than 1, which was lower than the control group. While B/E values in UC patients at remission stage and the control group were both larger than 1 , with no statistically significant difference between them. Conclusion There were obvious changes of intestinal bacterial flora in UC patients, and the change is more obvious in the UC patients at active stage, showing that there is a close relationship between intestinal mucosa asso-ciated bacterial flora and the development of UC.

Comparison of Bacterial Culture Rate and Bacterial Floral Distribution in Anophthalmic Patients with Prosthetic Eye, Between Patients with Symptom and Without Symptom

PURPOSE: To evaluate the distribution of conjunctival bacterial flora in anophthalmic socket patients with a prosthetic eye, and compare the bacterial positive culture rates between patients with subjective symptoms such as eye wax or irritation and patients without symptoms. METHODS: Twenty-six anophthalmic socket patients with a prosthetic eye who visited our clinic between December 2009 and May 2011 were retrospectively analyzed. The patients were asked about their symptoms, followed by a conjunctiva examination. Specimens were obtained from the inferior conjunctival cul- de- sac with a sterile cotton-tipped applicator. The collected specimens were cultured. RESULTS: The results indicated that the overall positive culture rate in the anophthalmic conjunctival socket was 69.2%, and the predominant organism was S. epidermidis (38.5%). Potential pathogenic bacteria were found in 4 eyes with a 15% positive culture rate. The incidence of bacteria was significantly higher (85.4%) in patient samples with subjective symptoms compared to patients without symptoms (50%). The bacterial positive culture rate of the potential pathogen bacteria in the group with symptoms was higher at 21%, but was not statistically significant. CONCLUSIONS: Performing a pathogen culture test is necessary for prosthetic eye patients who complain of their symptoms. Additionally, the proper antibacterial treatment should be performed according to the antibiotics sensitivity of cultured bacteria.