Résumé
Objective: To investigate the chemical constituents from the dried whole plants of Swertia bimaculata. Methods: The compounds were isolated and purified by various column chromatography, and their structures were elucidated on the basis of chemical and spectroscopic analyses. Results: Thirty compounds were isolated from the n-BuOH fraction of S. bimaculata EtOH extract and identified as 1,3,6-trihydroxy-4,7-dimethoxyxanthone (1), 1,8-dihydroxy-2,3,4,5-tetramethoxyxanthone (2), 1,3,5-trihydroxy-4- methoxyxanthone (3), 1,3-dihydroxy-5-methoxyxanthone (4), 1,3,5-trihydroxyxanthone (5), 1,3,7-trihydroxy-4-methoxyxanthone (6), 2-(3'-O-β-D-glucopyranosyl) benzoyloxygentisic acid (7), veratrilogenin (8), demethylbellidifolin (9), isobellidifolin (10), 1,3,8-trihydroxy-4,5-dimethoxyxanthone (11), 1,3-dihydroxy-4,5-dimethoxyxanthone (12), methylbellidifolin (13), 1-hydroxy-2,3,4,7-tetramethoxyxanthone (14), mangiferin (15), astragalin (16), isoquercitrin (17), nicotiflorin (18), rutin (19), isoorientin-7,3'-dimethylether (20), swertisin (21), swertiajaponin (22), isoorientin (23), isoscutellarein-8-methyl ether (24), 8-chrysoeriol (25), kaempferol (26), luteolin (27), quercetin (28), β-sitosterol (29), and oleanolic acid (30). Conclusion: Compound 1 is a new compound. named bimaculatone A. Compounds 2-7, 16-20, and 24-26 are firstly obtained from the plants of Swertia L., and compounds 2-11 and 16-28 are obtained from S. bimaculata for the first time.