Résumé
Objective To investigate the role of CD4~+CD25~+ Treg cells on the pathogenesis of ankylos- ing spondylitis(AS), and to study the machanism of tumor necrosis factor(TNF)-?blocker on the treatment of AS by detecting the number of CD4~+D25~+ Treg cells before and after the treatment. Methods The diagno- sis of 10 AS patients was made based on the 1984 modified New York criteria. The patients received subcuta- neou injection of recombinant human tumor necrosis factor receptor-Fc fusion protein(rhTNFR-Fc)(etaner- cept)50 mg weekly for 8 weeks and 10 heathy subjects were enrolled for control. The mononuclear cells were isolated from peripheral blood in beth patients and controls. The number of CD4~+CD25~+ T cells and CD4~+ CD25~(high)T cells and the expression of CTLA-4. were detected by flow cytometry. Results The proportion of CD4~+CD25~+ T cells(24?19)% in total CD4~+ T lymphocytes of peripheral blood and CD4~+CD25~(high)T/CD4~+ T (6?6)% from AS patients before treated with rhTNFR-Fc was higher than that in healthy volunteers and AS patients after treatment(P
Résumé
Objective:To investigate if CD4~+CD25~+ regrating T cells can suppress GVHD mediated by CD4~+ T cells in murine semi-allogeneic bone marrow transplantation model and try to figure out the mechanisms evolved.Methods:C3H(H-2~k) as donors, C3H?B6(H-2~ k/b) F1(recipients) were lethally irradiated and reconstituted with antibody T cell depleted bone marrow(ATBM), and divided into 4 groups: (A)No further treatment(n=10);(B)Infused 5?10~5 C3H CD4~+T cells(CD4,n=10);(C)Infused 5?10~5 CD4~+CD25~+Treg(CD25,n=5);(D)Infused 5?10~5 CD4~+T cells plus 5?10~5 CD4~+CD25~+Treg(CD4/CD25,n=6).Results:All mice in ATBM group, CD25 group and CD4/CD25 groups survived until 60 days without any GVHD symptoms, while all mice in CD4 group died in 10 days from GVHD.Conclusion:CD4~+CD25~+Treg cells did not cause GVHD and can prevent GVHD mediated by CD4~+ T cells while co-injected with CD4~+ T cells.