Résumé
Aims: The aim of this study is to determine the effect of blood storage using CPDA-1 on packed cell volume, methaemoglobin and oxyhaemoglobin in different ABO/Rhesus blood types donated by some residents of Port Harcourt, Rivers State, Nigeria. Study Design: This is a comparative study aimed at evaluating the effect of storage on the levels of methaemoglobin, oxyhaemoglobin and packed cell volume using CPDA-1. A total of eight donors were recruited with each sample obtained from the eight (8) known blood groups A+,B+,O+,AB+, A-,B-,O-,AB- and analysis of samples were in triplicate. The donors were adult males with age ranging from 35-45 years and they were apparently healthy and free from transfusion transmissible infections. The different blood group samples were stored for 30 days and samples for analysis were collected at 5 days interval. Place and Duration of Study: The study was conducted in Port Harcourt, Rivers State, Nigeria. All blood donors were residents of Port Harcourt. Blood donated was stored at Military Hospital Blood Bank, Port Harcourt, in a blood bag of 450 ml containing 63 ml of citrate phosphate dextrose adenine-1 (CPDA-1). The analysis was carried out at Rivers State University, Post Graduate Laboratory within March 1st to May 27th, 2019. Methodology: A total of eight (8) different ABO/Rhesus blood types (A+,B+,AB+,O+,A-,B-,AB- and O-) were collected and stored using a blood bank refrigerator at temperature of 4°C. Day 0 was taken to be control and 5 days intervals in-between to day 30 acted as the test. Packed cell volume was estimated using micro-haematocrit method while oxyhaemoglobin and methaemoglobin levels were estimated spectrophotometrically as described by Evelyn and Malloy. Results: The result showed a significant decrease in mean packed cell volume, oxyhaemoglobin and methaemoglobin levels compared to a higher mean of these parameters in the control; and these differences were statistically significant (p<0.05) across all blood groups under study. The decrease in values were as a result of haemolysis that occurs during storage. Conclusion: Storage of blood irrespective of the blood group type using CPDA-1 for 30 days indicates that there are “storage lesions”. This is attributed to red cell haemolysis and ageing of red blood cells. In general, all blood types showed no significant difference in their haematological (packed cell volume, methaemoglobin, oxyhaemoglobin) characteristic deterioration or storage lesion based on blood type differences. It is therefore necessary to state that storage lesion characteristics are the same irrespective of the blood type, and that fresh blood be transfused, and if blood is stored, prolonged storage beyond 10 days should be avoided.
Résumé
Aim: This study assessed the level of plasma haemoglobin concentration in CPDA-1 stored blood with a view to determine the extent of haemolysis during the 35 days storage period. Study Design: This is an observational and comparative case-control study. Place and Duration of Study: The study was conducted using healthy male donors residing in Port Harcourt. Analysis was carried out at the Blood Bank of Rivers State University Teaching Hospital, formerly Braithwaite Memorial Specialist Hospital (BMSH), Port Harcourt, Nigeria, from February 1st to March 8th, 2017. Methodology: Blood for transfusion was collected from prospective male blood donor found to be in good health, aged between 18 and 52 years, with haemoglobin level within the range of 13.5 g/dl – 16 g/dl, body weight within 55 kg – 75 kg, and body temperature within 37.0 to 37.50C / 99.50F, into plastic bags containing anticoagulant CPDA-1, and handled under strict sterile condition to prevent bacterial contamination. The blood was stored in a blood bank refrigerator with a constant temperature of +2 to +60C under proper inspection at intervals for colour, turbidity, haemolysis and clot formation. Two milliliters of the sample was collected aseptically at different interval days of collection from the blood bag and analyzed using the HemoCue photometer. Results: Results showed no significant changes in plasma haemoglogin from day 1, 5, and 10, while significant increase in haemolysis occurred from day 15, 20, 25, 30, and 35 (p = 0.000), a significant increase (p<0.05) in plasma haemoglobin was observed from day 15 to day 35 of storage. Conclusion: It is pertinent therefore to note that the use of CPDA-1 does not completely stop the changes that occur in RBC as there are several changes occurring in stored blood collectively called “storage lesions”. Therefore, it is advisable that blood should be transfused within 14 days of storage to avoid transfusion of blood products that has lost most of its benefits to recipients, and where possible whole blood should be processed and components separated before storage to reduce the level of non-viable red blood cells.
Résumé
O presente trabalho teve como objetivo avaliar e comparar as alterações bioquímicas e hemogasométricas do sangue total canino armazenado em bolsas CPDA-1 e CPD/SAG-M. Foram utilizados 14 cães machos, adultos e saudáveis, distribuídos em dois grupos com sete animais cada, dos quais foi obtido sangue para o estudo. No primeiro grupo (G1), o sangue foi armazenado em bolsas CPDA-1 e, no segundo grupo (G2), o sangue foi armazenado em bolsas CPD/SAG-M. As amostras foram analisadas para: potássio, sódio, glicose, proteína plasmática total, DPG, pH sangüíneo, pO2 , pCO2 e bicarbonato. Os momentos estabelecidos para as análises laboratoriais foram: D0: imediatamente após a coleta do sangue dos animais; D7: sete dias após a coleta; D14: quatorze dias após a coleta; D21: vinte e um dias após a coleta; D31: trinta e um dias após a coleta; D41: quarenta e um dias após coleta. A análise dos resultados permitiu concluir que a bolsa CPD/SAG-M apresentou melhor desempenho quando comparada à CPDA-1 após 41 dias de estocagem. O DPG, mesmo com pH inferior a sete, continua sendo produzido por, pelo menos, uma semana após a estocagem do sangue na bolsa.
The present study was aimed at evaluating and comparing the biochemical and haemogasometrics alterations of the total blood of dogs stored in bags CPDA-1 and CPD/SAG-M. 14 male's dogs, adult and healthful dogs, were divided in two groups, which one blood was removed for the study. The blood of the first group (G1) was stored in bag CPDA-1 and that of the second group (G2) in bag CPD/SAG-M. The samples were analyzed for: potassium, sodium, glucose, total plasmatic protein, DPG, pH, pO2 , pCO2 , and bicarbonate. The moments for the laboratory analyses were established: D0: immediately after the collection of blood; D7: seven days after the collection; D14: fourteen days after the collection; D21: twenty and one days after the collection; D31: thirty and one days after the collection; D41: forty one days after collect. The evaluation of the results allowed including that: the bag CPD/SAG-M presented advantage when compared with the CPDA-1 after 41 days of stored. The DPG, even with pH lower then 7, continues being produced for, at least, one week after the stocking of the blood in the bag.
Sujets)
Animaux , Mâle , Chiens , Sacs Plastiques pour Préservation du Sang , Conservation de sang/médecine vétérinaireRésumé
BACKGROUND: Current blood and blood components are prepared from 320ml or 400ml blood collection. The analytic values and standard values of blood and blood components were evaluated at Seo-Bu Blood Center, The Republic of Korea National Red Cross, Seoul, Korea. METHODS: Blood and blood components were analyzed with weight, specific gravity, content volume, RBC counts, WBC counts, platelets counts, hemoglobin, hematocrit, pH, total protein, albumin, factor VII and bacterial culture. RESLUTS: The volume, hemoglobin, hematocrit and pH of 320ml and 400ml standard unit of whole blood was 328 +/- 31ml and 405 +/- 22ml, 12.7 +/- 1.7g/dl and 14.8 +/- 2.0g/dl, 38.0 +/- 4.0% and 40.8 +/- 4.6%, mean of 7.16 and 7.13, respectively. The volume and hematocrit of packed red cells prepared from 320ml and 400ml standard unit of whole blood was 188 +/- 23ml and 248 +/- 23ml, 73.2 +/- 4.7% and 72.6 +/- 5.4%, respectively. Leukocytes poor red cells from 400ml standard unit of whloe blood showed 225 +/- 12ml of volume, 71 +/- 2.4% of hematocrit, and WBC removal was 87 +/- 5%. The volume, hematocrit and total protein in washed red cells was 224 +/- 11ml, 59 +/- 6.0% and 0.10 +/- 0.05g/unit, respectively. Leukocytes concentrates revealed 50 +/- 4.6ml of volume, 2.0 +/- 0.5x109/unit of WBC count and WBC recovery was 78 +/- 6.0%. Platelet concentrates prepared from 320ml and 400ml standard unit of fresh whole blood showed 38 +/- 3ml and 48 +/- 3ml of volume, 4.68 +/- 1.60x1010 and 5.55 +/- 1.80x1010 of platelet per unit, and 7.05 +/- 0.25 and 6.95 +/- 0.34 of pH, respectively. The fresh frozen plasma from 320ml standard unit of whole blood contained 143 +/- 25ml of volume, and that from 400ml whole blood showed 161 +/- 27ml of volume and 112 +/- 33 IU/unit of factor VII. The cryoprecipitate from 320ml whole blood showed 42 +/- 3ml of volume and 81 +/- 9 IU/unit of factor VII. There were no bacterial growth for all the components inoculated. CONCLUSION: At Seo-Bu Blood Center, we evaluated the characteristics of current blood and blood components, and established the standard values for whole blood (320ml, 400ml) as well as packed red cells, leukocytes poor red cells, washed red cells, leukocyte concentrates, platelet concentrates, fresh fozen plasma and cryoprecipates from 320ml and 400ml whole blood based on the present studies. Compared to the Standards for Blood Banks and Transfusion Services, American Association of Blood Banks, values of evluated items of current blood and blood components showed comparable results, but platelet counts from 320ml collection did not meet to those standards.