Résumé
Objective:To distinguish the differences between Olibanum and Myrrha by using modern analytical methods such as Differential Scanning Calorimeter (DSC) and Fourier Transform InfraRed (FT-IR). Methods:By collecting Olibanum and Myrrha in different growing areas and different processed prosecures, this paper to analizes the influence of temperature increase and its speed , as well as the particle size on the DSC experiment. The DSC method was used to perform a differential thermal map of Olibanum and Myrrha scanning and analysis; FT-IR was used to scan and analyze 20 batches of Olibanum and Myrrha. Results:TThe results of DSC analysis showed that the DSC experimental condition ranged between 30-600 ℃; the speed of temperature increase was 30 ℃/min; the particle size was 100 mesh. The DSC spectra of of Olibanum and Myrrha were significantly different. Only the processed products of frankincense had endothermic peak near 297 ℃, and there was no characteristic peak in this temperature range. Their exothermic peaks are close at 326 ℃, but their enthalpy values are quite different. The position of endothermic peak near 100 ℃ is close to the size of peak shape. FT-IR test showed that the absorption peaks of Olibanum and Myrrha at wave numbers 2 925, 1 710, 1 454, 1 371, 1 242, 1 029 cm -1 appeared, and the positions of strong peaks were also similar. The intensity of the characteristic peak of Myrrha wave number 1 029 cm -1 is greater than that of Olibanum. Conclusion:The DSC spectra of Olibanum and Myrrha are significantly different, and the difference between the two FT-IR spectra is small. Differential scanning Calorimetry is an effective, fast, and simple way to identify resinous Chinese medicinal materials, and is worthy of further popularization.
Résumé
Objective To explore a quick and simple method to identify ginseng medicinal materials. Methods Twenty types of ginseng medicinal materials were collected. The effects of two important factors:temperature and heating rate on differential scanning calorimetry (DSC) were investigated. The DSC features were used to quickly identify Radix et Rhizoma Notoginseng, Radix et Rhizoma Ginseng, Radix Panacis Quinquefolii and Panna ginseng C.A.Mey. Results The optimal experimental conditions for rapid identification of Radix et Rhizoma Notoginseng, Radix et Rhizoma Ginseng, Radix Panacis Quinquefolii and Panna ginseng C.A.Mey by DSC were determined: temperature range 30-400 ℃, heating rate 30 ℃/min. Under these conditions, the characteristic peak temperature and thermal enthalpy value can be used to distinguish four kinds of ginseng. Conclusions The experiment shows that the DSC method has the advantages of simple operation, rapid measurement, good reproducibility, easy analysis of the spectrum and less sample consumption. It is a new method for quickly identifying Chinese medicinal materials of ginseng genus.
Résumé
Objective To develop the ribavirin purity certified reference material( CRM ) which has measurement traceability and high accuracy,and establish an effective method of evaluation. Methods The homogeneity and the stability were checked by differential scanning calorimetry(DSC). High performance liquid chromatography(HPLC)and DSC methods were used for purity determination of ribavirin. Results Ribavirin showed satisfactory homogeneity and stability. The certified value of ribavirin was 99. 5%with an uncertainty of 0. 4%(k=2,P=0. 95). Conclusion Ribavirin purity CRM obtained in this paper has been proven to be a national primary CRM with high accuracy and traceability,which can be used to validate analytical methods,improve the accuracy of measurement data,establish meteorological traceability of analytical results as well as control the quality of ribavirin in the pharmaceutical industry.