RÉSUMÉ
Objective To investigate the expression and significance of detecting carbonic anhydrase Ⅸ(CAⅨ), pax-illin (paxillin) and somatostatin receptors (SSTR) in patients with duodenal carcinoma. Methods The immunohisto-chemistry was used to detect 87 cases of duodenal carcinoma carbonic anhydrase Ⅸ (CA Ⅸ), paxillin (paxillin) and somatostatin receptors (SSTR) expression. Results Among 87 cases of duodenal cancer, CA Ⅸ positive rate of 71.3%(62/87), paxillin positive rate of 74.7% (65/87), was significantly higher than other groups (P<0.05). The rate of so-matostatin receptor-positive rate of 48.2%(42/87),was significantly lower than than other groups (P<0.05). And the positive rate of duodenal CA Ⅸ poorly differentiated cancer group, the positive rate of protein pile, SSTR-positive rate compared with the well-differentiated group (P<0.05). The positive rate of M1 CA Ⅸ of duodenal papilla cancer, the positive rate of paxillin, SSTR-positive rate compared with M0 stage(P<0.05). Conclusion Carbonic anhydrase Ⅸ(CAⅨ), occurrence, development and metastasis of somatostatin receptor (SSTR) and paxillin and three closely related duodenal cancer, can serve as an important indicator of early diagnosis of cancer, can also be used to assess tumor development and prognosis.
RÉSUMÉ
Objective To investigate the pile protein (paxillin) and carbonic anhydrases Ⅸ (CA Ⅸ)expression and its significance in duodenal papilla carcinoma patients.Methods Eighty-seven cases of duodenal papilla carcinoma were enrolled as our subject.Immunohistochemical staining was used to detect the expressions of paxillin and CAⅨ in site of tumor,adjacent tissue and duodenal papillitis tissue.Results Sixtyfive cases (74.7％ (65/87)) were showed a higher expression of paxillin in cancer site than that of adjacent tissue (41％ (16/39),P ＜ 0.05) and duodenal papillitis tissue (41.3％ (19/46),P ＜ 0.05),and the difference was statisically significant (x2 =19.869,P ＜0.05).While a higher expression of CA Ⅸ in 62 cases (71.3％) in cancer site was seen,higher than that of adjacent tissue (33.3％ (13/39),P ＜ 0.05) and duodenal papillitis tissue (30.4％ (14/46),P ＜ 0.05),and the difference was statisically significant (x2 =26.936,P ＜ 0.05).As the increase of the cancer tissue malignant degree and the surrounding tissue invasion or distant metastasis degree,paxillin and CA Ⅸ expression increased.Conclusion The paxillin and CAⅨ might served as the early indicators for diagnosis and judgment of prognosis of duodenal papilla cancer.
RÉSUMÉ
Carbonic anhydrase(CA) is one of a group of enzyme that contains zinc. They exist widely in the mammals. CA has at least 14 species of isoenzyme. Among them,CAIX is located at the downstream of VHL tumor suppressor gene,and it can be activated through HIF-1 pathway. CAIX primarily exists in the mucous membrane of gastrointestinal tract in normal human tissues and its expression is low. However,higher expression of CAIX can be detected in renal cell carcinoma,cervical carcinoma and lung cancer,it may be closely related with the occurrence and development of the tumor. In addition,we can predict the prognosis of tumor patients by analysis of the expression of CAIX,thus,it is considered that CAIX is a tumor specifi c antigen. Due to its highly specifi c expression in renal cell carcinoma,CAIX will become the potential target antigen of tumor vaccine as well as the target gene for the treatment of RCC. The application of CAIX shows high promise in RCC target therapy. Hence,it is defi nitely important for us to investigate the expression and regulation of the CAIX.