RÉSUMÉ
Senescence is the major risk factor that promotes development of different stages of chronic liver diseases and is closely related to occurrence of hepatocellular carcinoma.Significant differences consist in clinicopathological features and tumor microenvironment between elderly and young patients with hepatocellular carcinoma.With rapid development of systemic therapy,immune checkpoint inhibitors combined with targeted therapy have greatly improved the prognosis of patients with advanced hepa-tocellular carcinoma.The selection of treatment decisions for elderly patients with hepatocellular carcinoma requires to consider u-nique age-related issues.Adequate communication and necessary evaluation should be carried out before making decisions.Elderly patients with hepatocellular carcinoma are speculated to benefit from combination immunotherapy based on age subgroup analysis of large clinical studies.However,data of effects and security obtained from clinical trials has certain limitations when being ap-plied in elderly populations of real world.The optimal therapeutic strategies for elderly patients with hepatocellular carcinoma still remain to be further explored in large-scale prospective clinical studies.
RÉSUMÉ
The ability of the human normal uterine cervix and cervical carcinoma to interconvert estradiol-17β and estrone is due to the activity of 17β-hydroxysteroid dehydrogenase (17β-HSD). Human cervical 800 x g supernatant fractions were analyzed by incubating with 3H-estradiol (20 nM) and NAD (0.25 ตmol) in 50 mM Tris buffer at pH 8.0. The incubation was terminated by adding 1 ml of 100% methanol and the supernatants were evaporated in air. The dry residues were resuspended in methanol, and aliquots were chromatographed in silica gel thin layer plates in benzene : ethanol (9 : 1, V : V). The 17β-HSD activity was determined from the amount of estrone formed and expressed as nanomoles of estrone formed/mg protein X h. The 17β-HSD activity was higher in normal cervix than in cervical carcinoma. Estradiol-17β added to incabation mixture caused a slight increase in enzyme activity only in cervical carcinoma while it had no effect in normal tissues. Progesterone administration increased 17β-HSD activity in some cases of normal and carcinoma cervix. These results suggest that changes in enzyme concentrations may play a physiologic role in the regulation of tissue levels of estradiol-17β.