Résumé
study the methylation status of caveolin-2(CAV2) gene in peripheral blood of uygur pigeon breeder lung patients, and discuss the significance of methylation of CAV2. Methods Twenty cases of uygur people who suffered from diseases after raising pigeons were enrolled into case group; 20 Urgur pigeons without disease were enrolled into case control group; and 20 uygur healthy subjects without pigeon were enrolled into healthy control group. General data of 60 subjects were collected and peripheral blood samples were collected. DNA was extracted from the retained peripheral blood samples, followed by hydrogen sulfite transformation, PCR amplification, in vitro transcription and RNase A-specific enzyme digestion, and finally, matrix-assisted laser desorption ionization time-of-flight mass spectrometry was used to detect the methylation of CAV2. Results CpG site of CAV2 fragment(CpG_1, CpG_2-4, CpG_5, CpG_6-8, CpG_9, CpG_10, CpG_11), actually detected 9 sites (CpG_1, CpG_2-4, CpG_5, CpG_6-8, CpG_11), the methylation rate distribution of each site in the three groups showed no statistical differences (P>0.05). The methylation rates of each site between the three groups were compared in pairs, and showed no statistical differences (P > 0.05). Conclusions Whether CAV2 gene methylation has any effect on the pulmonary pathogenesis and pulmonary fibrosis process of uygur pigeon feeders remains to be further studied.
Résumé
Objective@#To study the methylation status of caveolin-2(CAV2) gene in peripheral blood of uygur pigeon breeder lung patients, and discuss the significance of methylation of CAV2.@*Methods@#Twenty cases of uygur people who suffered from diseases after raising pigeons were enrolled into case group; 20 Urgur pigeons without disease were enrolled into case control group; and 20 uygur healthy subjects without pigeon were enrolled into healthy control group. General data of 60 subjects were collected and peripheral blood samples were collected. DNA was extracted from the retained peripheral blood samples, followed by hydrogen sulfite transformation, PCR amplification, in vitro transcription and RNase A-specific enzyme digestion, and finally, matrix-assisted laser desorption ionization time-of-flight mass spectrometry was used to detect the methylation of CAV2.@*Results@#CpG site of CAV2 fragment(CpG_1, CpG_2-4, CpG_5, CpG_6-8, CpG_9, CpG_10, CpG_11), actually detected 9 sites (CpG_1, CpG_2-4, CpG_5, CpG_6-8, CpG_11), the methylation rate distribution of each site in the three groups showed no statistical differences (P > 0.05). The methylation rates of each site between the three groups were compared in pairs, and showed no statistical differences (P > 0.05).@*Conclusions@#Whether CAV2 gene methylation has any effect on the pulmonary pathogenesis and pulmonary fibrosis process of uygur pigeon feeders remains to be further studied.
Résumé
Objective To analyze the differential expression of caveolin-2 in the psoriasis vulgaris and normal skin tissues, and investigate the relationship between caveolin-2 and the development of psoriasis vulgaris. Methods The expression of caveolin-2 mRNA and protein in psoriasis vulgaris patients and normal skin tissues were detected by quantitative PCR, immunohistochemistry and Western blot respectively. Results The quantitative PCR showed that the expression of caveolin-2 mRNA significantly decreased in the psoriasis vulgaris skin tissues when compared with the normal skin tissues (P < 0.01). The immunohistochemistry demonstrated that the caveolin-2 protein was mainly expressed in the cytoplasm of the basal layer cells in the normal skin tissues, but the caveolin-2 protein was not expressed in the lesions of psoriasis vulgaris. And the results of Western blot showed that the expression of caveolin-2 protein was significantly reduced in the psoriasis vulgaris skin tissues compared with the normal skin tissues. Conclusion The expression of caveolin-2 was reduced or lost in lesional epidermis of psoriasis vulgaris patients, which may serve as an aetiological factor in the development and or progression of psoriasis.
Résumé
Objective To investigate the effects of hyperbaric oxygen (HBO) on the expression of caveolin2 and matrix-metalloproteinase-9 (MMP-9) in brain tissues and the blood brain barrier (BBB) after cerebral ischemia and reperfusion (I/R) in rats. Methods Three hundred and seventy male Wistar rats were randomly assigned into sham,ischemia-reperfusion,HBO,and I/R + HBO groups. After creating cerebral I/R models,oxygen at 0.25 MPa was administered 5 times,and 2％ Evans blue (EB) was injected into the tail veins 1 h before the rats were sacrificed.The permeability of the BBB,the expression of caveolin-2 and MMP-9,and EB content were determined by Western blotting,immunohistochemistry,and spectrophotometery,respectively. Results In the I/R group,the EB content increased steadily to a peak at 4 hours.EB content in the IR + HBO group was significantly lower than in the I/R group.Caveolin-2 and MMP-9 were significantly augmented by I/R injury at the 24th,48th and 72nd hours.Compared to the I/R group,HBO intervention decreased their expression levels. Conclusion HBO intervention can reverse the increase of caveolin-2 and MMP-9 caused by I/R injury,which suggests a mechanism for protective effects of HBO on the permeability of the BBB in I/R injury.
Résumé
The organic anion transporters (OAT) have recently been identified. Although the some transport properties of OATs in the kidney have been verified, the regulatory mechanisms for OAT's functions are still not fully understood. The rat OAT1 (rOAT1) transports a number of negatively charged organic compounds between the cells and their extracellular milieu. Caveolin (Cav) also plays a role in membrane transport. Therefore, we investigated the protein-protein interactions between rOAT1 and caveolin-2. In the rat kidney, the expressions of rOAT1 mRNA and protein were observed in both the cortex and the outer medulla. With respect to Cav-2, the expressions of mRNA and protein were observed in all portions of the kidney (cortex < outer medulla = inner medulla). The results of Western blot analysis using the isolated caveolae-enriched membrane fractions or the immunoprecipitates by respective antibodies from the rat kidney showed that rOAT1 and Cav-2 co-localized in the same fractions and they formed complexes each other. These results were confirmed by performing confocal microscopy with immunocytochemistry using the primary cultured renal proximal tubular cells. When the synthesized cRNA of rOAT1 along with the antisense oligodeoxynucleotides of Xenopus Cav-2 were co-injected into Xenopus oocytes, the [14C]p-aminohippurate and [3H]methotrexate uptake was slightly, but significantly decreased. The similar results were also observed in rOAT1 over-expressed Chinese hamster ovary cells. These findings suggest that rOAT1 and caveolin-2 are co-expressed in the plasma membrane and rOAT1's function for organic compound transport is upregulated by Cav-2 in the normal physiological condition.