Résumé
Objective To study the liver protection of total celosins (CES) in different liver damage models .Method A-cute liver damage mouse model ,chronic liver damage rat model and immune liver damage mouse model were established for this study .Liver function tests ,liver lipid peroxidation indicators and liver pathology were observed .Results CES of each dose group can lower serum ALT and AST in different degrees ,can significantly reduce the content of MDA in liver homogenate , while can increase SOD activity .Pathological examination showed that animals administered significantly reduced the degree of liver cell damage .Conclusion CES have protective effects on acute liver damage induced by CCl 4 ,chronic liver damage induced by CCl4 and immunological liver damage induced by concanavalin A .Its hepatoprotective effect may be related to its antioxidant effects .
Résumé
Objective: To establish an analytical method for the simultaneous determination of celosin I and celosin II, two major triterpenoids in Celosia Semen and compare the contents of celosin I and celosin II from different habitats to screen the resources of elite germplasm for further applications. Methods: A sensitive and simple high-performance liquid chromatography coupled with evaporative light-scattering detector (HPLC-ELSD) method was developed for the first time for the simultaneous determination of celosin I and celosin II. Using this method, 21 batches of Celosiae Semen were determined from different habitats in China. Results: There was an obvious difference in the contents of celosin I and celosin II among Celosiae Semen species from various habitats across China. The crude drug from Yongzhou, Hunan province, Zhuhai, Guangdong province, and Nanning, Guangxi province showed the highest contents of all habitats, while Anguo, Hebei province, Haidian, Beijing, and Zhengzhou, Henan province showed the lowest content. The results also showed that geographical location had a great influence on the contents of the two compounds. The batches from lower latitudes were higher in contents of celosin I and celosin II. Conclusion: The sun light may be a key factor influencing the contents of the two saponins, indicating that the environment has a great impact on the quality of Celosiae Semen.