Résumé
Aim To explore the neuroproteetion of Chuanxiong injection on post-stroke depression (PSD) and its effeet on cAMP-p-CREB-BDNF pathway in hip¬pocampus.Methods Hats were randomly divided into four groups of sham, model, low-dose ( 0.1 g • kg ~1 ) of Chuanxiong injection and high-dose (0.2 g • kg 1 ) of it.The PSD model was established by middle cere-bral artery occlusion ( MCAO) combined with chronic stress stimulation.After the treatment, the behaviors of rats were observed by the tests of sugar consumption, open-field and Morris water maze.The function and status of neurons in hippocampus CA1 area were detec¬ted by Nissl staining and Neun immunofluorescence.The expressions of cAMP-p-CREB-BDNF pathway pro¬teins in hippocampus were observed by Western blot.Results Both Chuanxiong injection groups of low-dose and high-dose significantly increased consumption of sugar water, horizontal anrl vertical movement scores, decreased total route distance and route near the plat, elevated the number of Nissl body in CA1 area, re¬stored the morphology of Nissl body, and enhanced the expressions of cAMP and p-CREB in hippocampus.Additionally, the high dose group shortened swimming duration, reduced distance ratio of near plat to far from plat, and increased the expressions of Neun and BD- NF.Conclusions Chuanxiong injection has the po¬tential of improving the behavior and neurological func¬tion of PSD rats via up-regulating cAMP-CREB-BDNF pathway, protecting the neurons in hippocampal CA1 area, and ultimately alleviating the cognitive deficiency of PSD rats.
Résumé
Objective To study the Danshen Chuanxiong injection on the expression of serum inflammatory factors in patients with severe burn ,and the preventive effect on SIRS and multiple organ dysfunction syn-drome (MODS) .Methods 56 patients with the severe burn admitted in the hospital from January 2015 to Jan-uary 2016 were enrolled in the study ,and divided randomly into the control group and the observation group . The control group received routine treatment ,while the observation group received routine treatment as well as intra verous drip infusion treatment of Danshen Chuanxiong at the same time .The clinical efficacy in two groups and the incidence rates of SIRS and MODS were evaluated .The changes of the expression of serum C reactive protein (CRP) ,interleukin -10 (IL-10) and IL-6 before and after treatment ,and their correlations with the severity of SIRS and MODS was analyzed .Results The clinical efficacy of the observation group was better than that of the control group ,and the difference was statistically significant (P<0 .05) .The incidence rate of SIRS in the observation group was lower than that in the control group and the difference was statisti-cally significant (P<0 .01);the illness condition of the patients with SIRS in the observation group were less serious than those in the control group ,and the differences was statistically significant (P<0 .01) .There was no statistically significant difference in the incidence rates of MODS between the observation group and the control group (P>0 .05) ,but the conditions of MODS patients in the observation group were obviously lessheavier than those in the control group ,and the differences were statistically significant (P< 0 .05) .After treatment ,the indexes of IL-6 ,CRP and IL-10 were significantly improved ,and the differences were statistical-ly significant (P<0 .05) .After treatment ,IL-6 and CRP levels in the observation group were lower than those in the control group ,while the IL-10 level was higher than that in the control group ,and the differences were statistically significant ( P< 0 .05) .SIRS had a relatively high correlation with IL-6 ,CRP and IL-10 ( P<0 .05) ,and there was a relatively high correlation between MODS and IL-6 and CRP (P<0 .05) ,and there was a moderate correlation between MODS and IL-10 (P<0 .05) .Conclusion Danshen Chuanxiong injection can control the inflammatory response in patients with severe burns ,improve the expression level of serum IL-6 , CRP and IL-10 ,and it has a clear positive effect on preventing the occurrence of SIRS and MODS and allevia-ting the severity of SIRS and MODS ,which is worth of clinical attention .