RÉSUMÉ
Objective: To study the effect of compatibility on pharmacokinetics of the main components of Guizhi Zhumian prescription (GZP) based on the concept of Q-marker and to provide evidences for the determination of the Q-markers of this prescription. Methods: UPLC-MS/MS method was established for the study of the pharmacokinetics behaviors of seven main components, including cinnamic acid, 4-methoxycinnamic acid, coumarin, gardenoside, genipin, geniposidic acid, and chlorogenic acid, before and after the compatibility of GZP in plasma of cynomolgus monkeys. Results: Compared with the pharmacokinetics parameters of Cinnamomum cassia and Gardenia jasminoides, the AUC and Cmax of cinnamic acid, coumarin, geniposidic acid, and chlorogenic acid in GZP were increased obviously; The t1/2 of cinnamic acid, 4-methoxycinnamic acid, and coumarin were decreased; And the tmax of 4-methoxycinnamic acid, and geniposide were prolonged. Conclusion: Compatibility of GZP could significantly change the in vivo exposure status of the main components, and the seven main compounds were determined to be the Q-markers of GZP.
RÉSUMÉ
The effects of Cinnamon granules on pharmacokinetics of berberine in Rhizoma Coptidis granules in healthy male volunteers,and the compatibility mechanism of Jiao-Tai-Wan (JTW) composed of Rhizoma Coptidis granules and Cinnamon granules were investigated.The concentration of berberine in plasma of healthy male volunteers was determined directly by high performance liquid chromatography (HPLC) after an oral administration of Rhizoma Coptidis granules alone or combined with Cinnamon granules (JTW).The plasma concentration-time curves of berberine were plotted.The data were analyzed with Drug and Statistics (DAS) 2.0 pharmacokinetic program (Chinese Pharmacology Society)to obtain the main pharmacokinetic parameters.The results showed that the plasma concentration-time curve of berberine was described by a two-compartment model.The Cmax,Tmax,t1/2 and CLz/F of berberine in Rhizoma Coptidis granules were 360.883 μg/L,2.0 h,3.882 h,119.320 L·h-1·kg-1 respectively,and those of berberine in JTW were 396.124 μg/L,1.5 h,4.727 h,57.709 L·h-1·kg-1 respectively.It was suggested that Rhizoma Coptidis granules combined with Cinnamon granules could increase the plasma concentration of berberine,promote berberine absorption and lengthen the detention time of berberine in healthy male volunteers.