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With the changes of lifestyle, the incidence of colorectal cancer is increasing year by year.Though the surgical techniques and adjuvant therapy means are continuously improving, the overall prognosis of patients is still poor. Micrometastasis of colorectal cancer is the main reason leading to recurrence and metastasis. Timely detection of micrometastasis is meaningful to accurately determine the condition, formulate reasonable treatment and improve survival time. With the development of medical detection and sophisticated means, the micrometastasis of coloroctal cancer detection has become the research hot spots. In this paper, the current status of micrometastasis of colorectal cancer detection methods, detection means and choice of marker are introduced.
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0.05)Conclusion There are some free cancer cells in the prepheral blood of some patients with gastric cancer before operation. Pulling and stimulating tumor in the operation may increase cancer cell dissemination.Simple ligation of peripheral veins around stomach could not completely prevent cancer cell dissemination in the peripheral blood.
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Purpose:To study the expression of CK19 and CK2 0mRNA in peripheral blood patients with FRGO Stage ⅠA to ⅡA cervical carcinoma and to investigate its clinical significance. Methods:Using the reverse transcription polymerase chain reacti on(RT-PCR),CK19 and CK20mRNA expression was examined in peripheral blood fro m 250 patients with early cervical carcinoma before operation,50 Patients with benign gynecological tumors and 18 healthy volunteers. In 250 patients,possible correlations between clinical pathological factors were analyzed. Results:The positive expression rates of CK19 and CK20mRNA were 36% and 24% in 250 cervical carcinomas respectively,in comparison with 3.0% an d 0% with benign gynecological tumors and all subjects in healthy volunteers wer e negative; The expression of CK19 and CK20 mRNA were significantly correlated w ith lymph vascular space involvement,but was not associated with prognostic fac tors including stage,differentiation,pathological types ,lymph node metastasis ,bully tumor size . In patients with CK19 mRNA(+)/CK20 mRNA(+),the rate of lymp h node metastasis and vascular space involvement and recurrence outside the pelv is was significantly higher than that of patients with CK1R mNA9(-)/CK20 mRNA(- )(P
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Objective:To obtain some effective objective markers used to predict the early liver metastasis of colorectal tumor,the relationship of liver metastasis of colorectal tumor with associate detection three markers such as CK20mRNA、CD44V6 and PCNA was studied. Methods:The expression of CK20mRNA in portal venous blood from 30 colorectal cancer patients was detected by fluorescent quarto RT-PCR,and the results of CD44V6 and PCNA in colorectal cancer tissue were determined by means of immunohistochemistry, and then compared with control groups through statistics analysis. Results:The rate of positive expression of CK20mRNA in colorectal cancer patients' portal venous blood was obviously superior to the level of benign pathological changes controls(P
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Objective To study the association of abdominal cavity metastasis of colorectal cancer with the expressions of CK20 mRNA, CD44v6 and P53 and the related significance. Methods CK20 mRNA in peritoneal fluid was detected by reverse transcription polymerase chain reaction (RT PCR) and proteins of CD44v6 and P53 in cancer tissues were examined immunohistochemically in 50 cases of colorectal cancer. Results Significant correlation was found between CK20 mRNA and the expressions of CD44v6 and P53 in cancer tissues. Conclusion The detection of CK20 mRNA in peritoneal fluid plus examination of CD44v6 and P53 in cancer tissues are helpful for the prediction of abdominal cavity metastasis of colorectal cancer and post operation treatment.