Résumé
Objective To develop a method for simultaneous determination of three hydrophilic components and two lipophilic components in Radix et Rhizoma Salviae Miltiorrhizae. Methods The RP-HPLC method was performed by using a Welchrom C18 column(250 mm×4.6 mm,5 μm)with a mobile phase of acetonitrile (A)-0.1%phosphoric acid(B). The gradient elution program was as follows:0-15 min,10%→12%A;-35 min,12%→20%A;-45 min,20%→60%A;-65 min,60%→65%A;-80 min,65%→80%A;-90 min,10%A. The flow rate was kept at 1.0 mL?min-1 . The detection wavelength was set at 280 nm. The column temperature was 30 ℃ . Results A good linearity was obtained over 0.059 5-2.380 0 μg for tanshinol, 0.346 0-13. 840 0 μg for rosmarinic acid, 0. 656 0 - 26. 240 0 μg for salviamolic acid B, 0. 420 0 - 16.800 0 μg for cryptotanshinone and 0.414 0- 16.560 0 μg for tanshinoneIIA, respectively ( r = 0.999 9). The average recovery rates were between 98.69%-100.91% with RSD less than 1.2%(n = 6). Conclusion The method is rapid, accurate, credible and repeatable, and can provide basis for the quality control of Radix et Rhizoma Salviae Miltiorrhiza.
Résumé
Objective: To develop a new method for the simultaneous determination of two hydrophilic components and two lipophilic components of Radix et Rhizoma Salviae Miltiorrhizae. Methods: The HPLC-DAD method was employed using a column of Agilent Zorbax TC C18 (4.6 mm × 250 mm, 5 μm) with a mobile phase of methanol -2% acetic acid. The gradient elution program was as follow:0-15 min, 30% B-40% B; 15-20 min, 40% B-60% B; 20-25 min, 60% B-90% B; 25-40 min, 90% B. The detection wavelength was set at 281 nm and the temperature was 35°C. Results: The linearity was obtained over 3.76-120.20 μg · ml-1 (r=0.999 9) for rosmarinic acid, 34.20-109 4.5 μg · ml-1 (r=0.999 9) for salviamolic acid B, 0.64-20.32 μg · ml-1 (r=0.999 9) for clyptotanshinon, and 1.02-32.72 μg · ml-1 (r=0.999 6) for tanshinone II A. The RSDs of precision and stability of the sample were both less than 1% in 48 hours. The average recovery was between 99.72%-100.63%. Conclusion: The present method is simple and has satisfactory efficacy; it can simultaneously determine multiple hydrophilic and lipophilic bioactive components in Salvia miltiorrhiza from different areas.