Effect of tumor cell component on the inducing macrophage cytotoxic factor / 中国免疫学杂志

In this study,we presented data that K562,YAC-1 and LAC-1 cells or tumor-membrane-associated protein could stimulate BCG-treated macrophages from BALB/c mice to produce cy-totoxic factor (M?-CF). CF-inducing activity of K562 membrane or tumor cells remainde afterheat-treated at 100℃ for 20 min, but decreased by trypsin digestion or NaIO_4 oxidixa-tion. Thisactivity was also hibited by ConA or D-Man, but not by Gal. and Giu. The results suggested thatgiycoprotein containing D-Man on tumor cell surface was responsible for traggering M?s toproduce CF.