Résumé
Members of the bZIP transcription factor family play crucial roles in the regulation of plant development, biosynthesis of secondary metabolites, and response to abiotic and biotic stresses. To date, multiple bZIPs have been identified and investigated in numerous plant species. However, few studies have characterized bZIPs from Dimocarpus longan Lour. In this study, nine bZIPs from D. longan were identified from RNA-Seq data and further verified using the NCBI conserved domain search tool and Pfam database. Bioinformatics tools were used to systematically analyse the physicochemical properties, protein structures, multiple sequence alignment, motif compositions, evolutionary relationships, secondary structures, subcellular localization, phosphorylation sites, signal peptides, GO annotations and protein–protein interactions of the DlbZIPs. The expression patterns of the nine DlbZIPs were evaluated by qRT-PCR in roots and leaves and in response to varying durations of a 38C heat treatment. DlbZIP3, DlbZIP5, DlbZIP6 and DlbZIP7 were differentially expressed between root and leaf tissues. All nine DlbZIPs responded to heat treatment in both roots and leaves, but their specific expression levels differed. DlbZIP4 and DlbZIP8 were highly expressed in roots after heat treatment, whereas DlbZIP1 and DlbZIP5 were highly expressed in leaves after heat treatment. These findings lay a foundation for increasing active secondary metabolite content and improving abiotic stress tolerance in D. longan using transgenic technology
Résumé
This study was aimed to create a method for content determination of quercetin, luteolin and kaempferol in flowers of Dimocarpus longan Lour. from different habitats of Guangxi province by HPLC. The samples were sepa-rated on the Hypersil C18 column (250 mm í 4.6 mm, 5 μm) which was eluted with methanol-0.2% phosphoric acid solution (47:53) with detective wavelength at 360 nm, flow rate at 1.0 mL·min-1, and column temperature at 30℃. The results showed that this method had a good linear relationship within the range of 0.18 to 2.88 μg for quercetin, 0.059 to 0.944 μg for luteolin, and 0.024 to 0.384 μg for kaempferol (r = 0.9999). The average recovery rate was 100.06% (RSD = 1.72%), 99.77% (RSD = 1.18%) and 98.67% (RSD = 1.99%, n = 9), respectively. It was conclud-ed that this method is simple, rapid, reliable and with good repeatability, which can be used in the quality control of flowers of Dimocarpus longan Lour.
Résumé
Objective: To investigate the chemical constituents of the fruits of Dimocarpus longan. Methods: The chemical constituents were isolated from 95% alcohol-extract on seeds of longan by silica gel, polyamide, as well as Sephadex LH-20 column chromatography. Their structures were identified on the basis of physical and chemical properties and spectral analysis. Results: Twelve compounds were isolated and identified as: β-sitosterol (1), 2-phenylethanol (2), 2-methyl-1,10-undecanediol (3) (24R)-6β-hydroxy-24- ethyl-cholest-4-en-3-one (4), oleanolic acid (5), pinoresinol (6), nicotinic acid (7), 4-hydroxybenzoic acid (8), β-daucosterol (9), 1-O-methyl-D-myo-inositol (10), uracil (11), and adenosine (12). Conclusion: Compounds 2-8 and 10 are reported from the pericarp of longan (the fruits of D. longan) for the first time, and compound 3 is a new compound named longandiol.
Résumé
Objective: To investigate the chemical constituents of pericarp of longan (Dimocarpus longan) fruits. Methods: The chemical constituents were isolated by silica gel, polyamide, as well as Sephadex LH-20 column chromatography and their structures were identified on the basis of physical and chemical properties and spectral analysis. Results: Thirteen compounds were isolated and identified as: friedelin (1), friedelanol (2), (24R)-stigmast-4-en-3-one (3), β-sitosterol (4), β-(2-furyl) acrylic acid (5), 6-hydroxy-7- methoxycoumarin (6), β-daucosterol (7), gallic acid (8), corilagin (9), heptyl p-hydroxybenzoate (10), methyl gallate (11), 4-O-α-L- rhamnopyranosyl-ellagic acid (12), and ellagic acid (13). Conclusion: Compounds 3-7, 10, and 12 are reported from the pericarp of longan fruits for the first time, and this is the first report for compound 5 as a natural product.