Résumé
Background @#We have done dynamic study of tannin and total flavonoid contents in samples of Iridaceae such as Iris bungei maxim, Iris tenuifolia Pall, Iris lactea Pall and Iris dichotoma from 2017 April to 2017 Oct. Those 4 samples were prepared from Khuld sum of Dundgobi, Tsogttsetsii sum of Umnugobi, Altanbulag soum of Tuv province and the Botanical Garden 2 of Medicinal plants of Drug Research Institute. @*Goal@#The purpose of this study is to determine how does change biological active compounds in Iridaceae species that depending on growing season and to describe which harvesting period is efficient to use. @*Materials and Methods@#The chemical contents were analyzed with the colorimetric assay, volume metric analysis method and spectrophotometric method.@*Result@#In result of study, there was shown differences in bioactive substance of each Iridaceae, overall content of polyphenolt compound and total flavonoid highest from April to June. But, the bioactive substance in the Iris dichotoma was the highest in August and constantly increased in May to August then constantly decreased in September and October. Comparisons between Iridaceae; the highest concentration of bioactive substance was Iris tenuifolia Pall and the lowest concentration bioactive substance was Iris lactea Pall.
Résumé
The authors has investigated the effect of intracisternal urokinase on the multihemorrhage canine model of chronic post-subarachnoid hemorrhage(SAH) hydrocephalus. Each of 16 adult mongrel dogs was assigned to one of two experimental groups. All animals received a total of 13ml of fresh unheparinized autologous blood via three cisternal injections. Eight animals were treated by intracisternal injection of 20,000 IU of Urokinase every 12 hours for 3 days, and the remaining were not treated. The changes in ventricular volumes were measured by computed tomography(CT) before and 3 months after the initial subarachnoid blood injection. To compare the changes of hydrodynamic properties in chronic phases of post-SAH hydrocephalus, the pressure-volume index(PVI) technique of bolus manipulation of cerebrospinal fluid(CSF) was used to measure the volume-buffering capacity of neural axis and the resistance to the absorption of CSF(before SAH, post-SAH 1 month, 3 months). The final ventricular volume at 3 months of control group was 4 times greater than the initial volume, but Urokinase group less than two times. The mean measured PVI values of control group and Urokinase group were 3.98+/-0.76ml(+/- standard deviation(SD)) and 4.01+/-0.82ml in baseline study, 3.09+/-0.96ml and 3.70+/-0.84ml in post-SAH 3 months. The mean resistance of CSF outflow of control group and Urokinase group were 10.30+/-2.24mm Hg/ml/min), and 10.34+/-1.98mm Hg/ml/min in baseline study. At 1 month and 3 months after SAH control group maintained high absorptive resistance(29.54+/-11.50mm Hg/ml/min, 22.43+/-3.82mm Hg/ml/min), whereas the resistances of Urokinase group were slightly increased and then returned to the original levels(16.04+/-4.87mm Hg/ml/min, 12.87+/-3.06mm Hg/ml/min). The results described in this experimental study indicated that if fibrinolysis of the subarachnoid blood clot can be achieved rapidly after SAH, the complicating chronic hydrocephalus might be prevented.