RÉSUMÉ
@#[Abstract] Objective: To explore the effects of triptolide on immune function and tumor cell proliferation in patients with cervical cancer. Methods: Sixty-two patients with cervical cancer admitted in the Department of Obstetrics and Gynecology of the Second Affiliated Hospital of Hebei North College between July 2015 and April 2018 were randomly divided into the control group (n=31) and the observation group (n=31). All patients received routine treatment after laparoscopy, while those in the observation group received additional triptolide. The treatment efficacy, serum immune cells, inflammatory factors and the levels of cyclinD1, estrogen receptor α (ERα ) were observed and compared between the two groups. Results: The total remission rate of the patients in the observation group was 87.10%, significantly higher than 61.29% in the control group (P<0.05). After treatment, the levels of CD3+ and CD4+ T lymphocytes and CD4+ /CD8+ T lymphocytes in the two groups increased significantly, with more obvious increase in the observation group than that in the control group (P<0.01). The levels of CD8+ and programmed cell death-ligand 1 (PD-L1) T lymphocytes in the two groups decreased significantly after treatment, with a more obvious decrease in observation group than that in control group (P<0.01). After treatment, the levels of interleukin-6 (IL-6), IL-10 and tumor necrosis factor-α (TNF-α) in the two groups decreased, and those in the observation group were significantly lower than those in the control group (P<0.05). After treatment, the positive expression rate of cyclinD1 decreased and the positive expression rate of ER α increased in both groups (all P<0.05), with no significant difference between the two groups (all P>0.05). Conclusion: On the basis of routine surgical treatment, triptolide can effectively improve the immune function, reduce the inflammatory response, inhibit the proliferation of tumor cells and regulate the expression of cancer-related factors in patients with cervical cancer, which has a certain therapeutic effect on cervical cancer.
RÉSUMÉ
Crop productivity and yield are adversely affected by abiotic and biotic stresses. Therefore, finding out the genesresponsible for stress tolerance is a significant stride towards crop improvement. A gene co-expression network is apowerful tool to detect the most connected genes during heavy metal (HM) stress in plants. The most connected genes maybe responsible for HM tolerance by altering the different metabolic pathways during the biotic and abiotic stress. In thesame line we have performed the GSE86807 microarray analysis of chickpea during exposure to chromium, cadmium andarsenic and analyzed the data. Common differentially expressed genes (DEGs) during exposure to chromium, cadmium andarsenic were identified and a co-expression network study was carried out. Hub and bottleneck genes were explored on thebasis of degree and betweenness centrality, respectively. A gene set enrichment analysis study revealed that genes likehaloacid dehydrogenase, cinnamoyl CoA reductase, F-box protein, GDSL esterase lipase, cellulose synthase, b-glucosidase13 and isoflavone hydroxylase are significantly enriched and regulate the different pathways like riboflavin metabolism,phenyl propanoid biosynthesis, amino acid biosynthesis, isoflavonoid biosynthesis and indole alkaloid biosynthesis.