Résumé
Objective: To study the chemical constituents from the petroleum ether and ethyl acetate extract of the twig and leaves of Taxus media. Methods: Several column chromatography including silica gel, Sephadex LH-20 gel, ODS and so on were applied in isolation and purification. The structures were elucidated on the basis of physicochemical properties and spectral data. Results: Eight taxane diterpenoids, five flavonoids, three phenolic acids, and two other compounds were isolated and determined as taxinine-11,12-oxide (1), 2-deacetoxytaxuspine C (2), 2-deacetoxytaxinine E (3), 7,9-deacetyltaxinine B (4), taxagifin (5), 9-deacetyltaxinine A (6), 9-deacetyltaxinine (7), 10-desacetylbaccatin III (8), kaempferol (9), aromadendrin (10), apigenin (11), sciadopitysin (12), ginkgetin (13), 4-hydroxy-benzaldehyde (14), p-hydroxybenzoic acid (15), pyrocatechol (16), β-sitosterol (17), and eicosan-10-ol (18), respectively. Conclusion: Compounds 1-4, 6, 7, 10, 14-16 are firstly isolated from the plant of T. media, and compound 18 is obtained from the Taxaceae for the first time.