Résumé
Objective@#To investigate the inhibitory effect of polydopamine (PDA) on enamel demineralization in isolated teeth and the induction of hydroxyapatite (HA) production on the surface of demineralized enamel to provide a novel protocol for the prevention and treatment of enamel demineralization. @*Methods@#Twenty isolated bovine teeth were cut into 20 enamel slices and randomly divided into an experimental group and a control group, with 10 slices in each group. The enamel slices in the experimental group were immersed in 2 mg/mL freshly prepared dopamine solution and incubated for 24 hours at room temperature in the dark to prepare the PDA coating, while the control group was left untreated. Then, the isolated bovine teeth, with and without PDA coating, were immersed in artificial demineralization solution at 37 °C for 3 days, followed by 7 days in simulated body fluid (SBF), and the immersion solution was changed daily. The surface morphology of enamel was observed by scanning electron microscopy (SEM), the calcium/phosphorus ratio of the enamel surface was analyzed by energy dispersive spectroscopy (EDS), and the characteristic functional groups in enamel deposits were analyzed by Fourier transform infrared spectroscopy (FTIR).@* Results@#Compared with the control group, the number of demineralized pores produced after 3 d of enamel demineralization with polydopamine coating was less, and the diameter was smaller. EDS elemental analysis showed that the Ca/P ratio after enamel demineralization was 2.37 in the experimental group, which was smaller than the 2.53 ratio in the control group. In the remineralization experiment, after 7 days of remineralization of PDA coated enamel in the experimental group, lamellar grains were produced on the enamel surface, and the growth showed obvious directionality, growth regularity and uniform arrangement. In the control group, the surface of enamel was flocculent mineral deposit, and the crystallinity was poor. The FTIR results proved that the enamel surface deposit of PDA-coated enamel was HA after 7 d of remineralization. @*Conclusion @#PDA can affect the nucleation process of HA and promote the production of HA on the surface of demineralized enamel.
Résumé
Introducción: en la práctica clínica de la estomatología es común encontrar profesionales que no conocen ni saben aplicar la clasificación de preparaciones cavitarias de Mount y Hume. En la literatura en idioma español es escasa la información acerca del tema, a pesar de que las entidades rectoras del gremio internacional la consideran vigente. Objetivo: comparar la clasificación de las preparaciones cavitarias dentales según Mount y Hume, modificada por Lasfargues y otros, con la de Black. Métodos: se realizó un estudio de revisión bibliográfica sobre la clasificación de las preparaciones cavitarias según Mount y Hume, en la que se incluyeron artículos publicados desde 2006 hasta 2014. Se revisaron revistas de impacto en Internet que tratan el tema (en: Google académico, Scopus, Scielo, Hinari, y Medline). Los términos utilizados fueron: new cavity classification, cavity design, preservation and restoration of tooth structure, enamel remineralization, Mount Hume Lasfargues Classification. Los 163 artículos obtenidos fueron tamizados; pero el estudio se ciñó solo a 52 artículos que trataban estas temáticas de manera más integral. Análisis e integración de la información: se consideró a la clasificación de Black como insuficiente en cuanto a las nuevas tecnologías y conceptos actuales orientadas al logro de terapias mínimamente invasivas. Mount y Hume demuestran con su clasificación las limitaciones de las preparaciones de Black. Consideraciones finales: la clasificación de Black presentó limitaciones en relación con la de Mount y Hume, y Lasfargues. Ambas clasificaciones deben coexistir, y es factible aplicarlas en la estomatología conservadora actual(AU)
Introduction: in dental practice it is common to find professionals who do not know or are unable to apply Mount & Hume's cavity preparation classification. Information about the subject is scarce in the literature published in Spanish, despite the fact that leading dental institutions worldwide consider it to have current validity. Objective: compare Mount & Hume's classification of dental cavity preparations as modified by Lasfargues and colleagues, with Black's. Methods: a bibliographic review was conducted of papers about Mount & Hume's classification of cavity preparations published from 2006 to 2014. The review included high impact online journals from the databases Google Scholar, Scopus, Scielo, Hinari and Medline. The search terms used were new cavity classification, cavity design, preservation and restoration of tooth structure, enamel remineralization, Mount Hume Lasfargues Classification. The 163 papers obtained were scanned, but the study was restricted to the 52 which dealt with the topic in a more comprehensive manner. Data analysis and integration: black's classification was considered to be insufficient in the light of the new technologies and current concepts about minimally invasive therapies. Mount & Hume's classification shows the limitations of Black's preparations. Final considerations: black's classification had limitations with relation to Mount & Hume's and Lasfargues'. Both classifications should coexist and it is feasible to apply them in current conservative dental practice(AU)